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Recombinant Glycoproteins

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Steven B. Bradfute

    1. Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2762)

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About this book

This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins.

Keywords

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Viral Surface Glycoproteins

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells

      • Annalisa Meola, Pablo Guardado-Calvo
      Pages 3-16

    3. Production and Purification of Filovirus Glycoproteins

      • Madeleine Noonan-Shueh, M. Javad Aman, Shweta Kailasan
      Pages 17-25

    4. Modification of N-Linked Glycan Sites in Viral Glycoproteins

      • Nicholas J. Lennemann, Lochlain Corliss, Wendy Maury
      Pages 27-41

    5. Production of Influenza Virus Glycoproteins Using Insect Cells

      • Madhumathi Loganathan, Benjamin Francis, Florian Krammer
      Pages 43-70

    6. SARS-CoV-2 S-Protein–Ace2 Binding Analysis Using Surface Plasmon Resonance

      • Jason Baardsnes, Béatrice Paul-Roc
      Pages 71-87

    7. A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain

      • Catherine Forest-Nault, Izel Koyuturk, Jimmy Gaudreault, Alex Pelletier, Denis L’Abbé, Brian Cass et al.
      Pages 89-105

  3. Protozoal and Nematode Glycoproteins and Polysaccharide Conjugates

    1. Front Matter

      Pages 107-107
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    2. Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis

      • Mohammad Naghizadeh, Susheel K. Singh, Jordan Plieskatt, Ebenezer Addo Ofori, Michael Theisen
      Pages 109-121

    3. Analysis of Caenorhabditis Protein Glycosylation

      • Katharina Paschinger, Jorick Vanbeselaere, Iain B. H. Wilson
      Pages 123-138

    4. Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates

      • Federico Urbano-Munoz, Caitlyn E. Orne, Mary N. Burtnick, Paul J. Brett
      Pages 139-148

  4. Mammalian Glycoproteins

    1. Front Matter

      Pages 149-149
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    2. Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors

      • Phuc Phan, Shivakumar Sonnaila, Gaetane Ternier, Oshadi Edirisinghe, Patience Salvalina Okoto, Thallapuranam Krishnaswamy Suresh Kumar
      Pages 151-181

    3. Production and Purification of Antibodies in Chinese Hamster Ovary Cells

      • Lauren Stuart
      Pages 183-190

    4. Mammalian Antigen Display for Pandemic Countermeasures

      • Andrea Quezada, Ankur Annapareddy, Kamyab Javanmardi, John Cooper, Ilya J. Finkelstein
      Pages 191-216

  5. Analysis of Glycoproteins

    1. Front Matter

      Pages 217-217
      Download chapter PDF

    2. Analysis of Native and Permethylated N-Glycan Isomers Using MGC-LC-MS Techniques

      • Andrew I. Bennett, Oluwatosin Daramola, Md Mostofa Al Amin Bhuiyan, Vishal Sandilya, Yehia Mechref
      Pages 219-230

    3. Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

      • Cristian D. Gutierrez Reyes, Akeem Sanni, Moyinoluwa Adeniyi, Damir Mogut, Hector R. Najera Gonzalez, Parisa Ahmadi et al.
      Pages 231-250

    4. Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

      • Cristian D. Gutierrez Reyes, Akeem Sanni, Damir Mogut, Moyinoluwa Adeniyi, Parisa Ahmadi, Mojgan Atashi et al.
      Pages 251-266
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Editors and Affiliations

  • Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, USA

    Steven B. Bradfute

Bibliographic Information

  • Book Title: Recombinant Glycoproteins

  • Book Subtitle: Methods and Protocols

  • Editors: Steven B. Bradfute

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3666-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3665-7Published: 06 February 2024

  • Softcover ISBN: 978-1-0716-3668-8Due: 19 February 2025

  • eBook ISBN: 978-1-0716-3666-4Published: 05 February 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 355

  • Number of Illustrations: 12 b/w illustrations, 77 illustrations in colour

  • Topics: Chemistry/Food Science, general

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