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Article
Open AccessLipidome characterisation and sex-specific differences in type 1 and type 2 diabetes mellitus
In this study, we evaluated the lipidome alterations caused by type 1 diabetes (T1D) and type 2 diabetes (T2D), by determining lipids significantly associated with diabetes overall and in both sexes, and lipid...
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Article
Open AccessEstablishing a framework for best practices for quality assurance and quality control in untargeted metabolomics
Quality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. These important practices will strengthen thi...
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Article
Metabolomics 2022 workshop report: state of QA/QC best practices in LC–MS-based untargeted metabolomics, informed through mQACC community engagement initiatives
The Metabolomics Quality Assurance and Quality Control Consortium (mQACC) organized a workshop during the Metabolomics 2022 conference.
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Article
A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenoty** (MAP/UK)
Targeted metabolite assays that measure tens or hundreds of pre-selected metabolites, typically using liquid chromatography–mass spectrometry, are increasingly being developed and applied to metabolic phenotyp...
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Article
Providing metabolomics education and training: pedagogy and considerations
Metabolomics is a highly multidisciplinary and non-standardised research field. Metabolomics researchers must possess and apply extensive cross-disciplinary content knowledge, subjective experience-based judge...
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Article
Open AccessQuality assurance and quality control reporting in untargeted metabolic phenoty**: mQACC recommendations for analytical quality management
Demonstrating that the data produced in metabolic phenoty** investigations (metabolomics/metabonomics) is of good quality is increasingly seen as a key factor in gaining acceptance for the results of such st...
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Article
Open AccessReference materials for MS-based untargeted metabolomics and lipidomics: a review by the metabolomics quality assurance and quality control consortium (mQACC)
The metabolomics quality assurance and quality control consortium (mQACC) is enabling the identification, development, prioritization, and promotion of suitable reference materials (RMs) to be used in quality ...
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Article
Chorioamnionitis alters lung surfactant lipidome in newborns with respiratory distress syndrome
Chorioamnionitis is associated with preterm delivery and morbidities; its role in lung disease is controversial. The aim of this study is to assess the effect of chorioamnionitis on metabolite and lipid profil...
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Article
Open AccessPerturbations in cardiac metabolism in a human model of acute myocardial ischaemia
Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to defin...
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Article
Correction to: Metabolic characterisation of disturbances in the APOC3/triglyceride‑rich lipoprotein pathway through sample‑based recall by genotype
As a result of incorrect processing of two raw datasets presented (LIPIDS-POS and LIPIDS-NEG), some metabolite annotations reported in the original publication were incorrectly labeled. Correction of this erro...
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Article
Open AccessCytoglobin protects cancer cells from apoptosis by regulation of mitochondrial cardiolipin
Cytoglobin is important in the progression of oral squamous cell carcinoma but the molecular and cellular basis remain to be elucidated. In the current study, we develop a new cell model to study the function ...
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Article
Dissemination and analysis of the quality assurance (QA) and quality control (QC) practices of LC–MS based untargeted metabolomics practitioners
The metabolomics quality assurance and quality control consortium (mQACC) evolved from the recognized need for a community-wide consensus on improving and systematizing quality assurance (QA) and quality contr...
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Article
Open AccessMetabolic characterisation of disturbances in the APOC3/triglyceride-rich lipoprotein pathway through sample-based recall by genotype
High plasma triacylglyceride levels are known to be associated with increased risk of atherosclerotic cardiovascular disease. Apolipoprotein C-III (apoC-III) is a key regulator of plasma triacylglyceride level...
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Article
Open AccessMultiple metabolic pathways are predictive of ricin intoxication in a rat model
Exposure to ricin can be lethal and treatments that are under development have short windows of opportunity for administration after exposure. It is therefore essential to achieve early detection of ricin expo...
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Article
Towards quality assurance and quality control in untargeted metabolomics studies
We describe here the agreed upon first development steps and priority objectives of a community engagement effort to address current challenges in quality assurance (QA) and quality control (QC) in untargeted ...
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Protocol
Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer
Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most frequently applied analytical platform in the untargeted metabolomic study of mammalian urine. Here we describe two complementary...
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Article
Open AccessGuidelines and considerations for the use of system suitability and quality control samples in mass spectrometry assays applied in untargeted clinical metabolomic studies
Quality assurance (QA) and quality control (QC) are two quality management processes that are integral to the success of metabolomics including their application for the acquisition of high quality data in any...
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Article
Open AccessExercise and high-fat feeding remodel transcript-metabolite interactive networks in mouse skeletal muscle
Enhanced coverage and sensitivity of next-generation ‘omic’ platforms has allowed the characterization of gene, metabolite and protein responses in highly metabolic tissues, such as, skeletal muscle. A limitat...
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Article
Quality assurance and quality control processes: summary of a metabolomics community questionnaire
The Metabolomics Society Data Quality Task Group (DQTG) developed a questionnaire regarding quality assurance (QA) and quality control (QC) to provide baseline information about current QA and QC practices app...
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Chapter
Collection and Preparation of Clinical Samples for Metabolomics
A wide range of biofluids (urine, serum, plasma, saliva, etc.) as well as cellular and tissue samples can be collected and investigated in clinical metabolomic studies. The choice of sample is dependent on the...
