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Article
Natural antisense transcripts as versatile regulators of gene expression
Long non-coding RNAs (lncRNAs) are emerging as a major class of gene products that have central roles in cell and developmental biology. Natural antisense transcripts (NATs) are an important subset of lncRNAs ...
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Protocol
Site-Directed Mutagenesis Protocol to Determine the Role of Amino Acid Residues in Polycomb Group (PcG) Protein Function
Site-directed mutagenesis (SDM) is a technique in molecular biology and protein engineering that is widely used to determine the significance of specific residues involved in post-translational modifications (...
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Article
Open AccessAutoregulation of JARID2 through PRC2 interaction with its antisense ncRNA
JARID2 is a member of chromatin-modifying Polycomb Repressive Complex-2 or PRC2. It plays a role in recruiting PRC2 to developmental genes and regulating its activity. JARID2 along with PRC2 is indispensable f...
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Article
Open AccessExercise and high-fat feeding remodel transcript-metabolite interactive networks in mouse skeletal muscle
Enhanced coverage and sensitivity of next-generation ‘omic’ platforms has allowed the characterization of gene, metabolite and protein responses in highly metabolic tissues, such as, skeletal muscle. A limitat...
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Protocol
Identification of RNA–Protein Interactions Through In Vitro RNA Pull-Down Assays
Recent advances in next-generation sequencing have revealed that majority of the human genome is transcribed into long and short RNA (ncRNA) transcripts. Many ncRNAs function by interacting with proteins and f...
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Article
Open AccessMicro-Fresnel-Zone-Plate Array on Flexible Substrate for Large Field-of-View and Focus Scanning
Field of view and accommodative focus are two fundamental attributes of many imaging systems, ranging from human eyes to microscopes. Here, we present arrays of Fresnel zone plates fabricated on a flexible sub...
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Article
Open AccessT-bet and GATA3 orchestrate Th1 and Th2 differentiation through lineage-specific targeting of distal regulatory elements
T-bet and GATA3 regulate the CD4+ T cell Th1/Th2 cell fate decision but little is known about the interplay between these factors outside of the murine Ifng and Il4/Il5/Il13 loci. Here we show that T-bet and GATA...
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Article
Open AccessNoncoding RNA localisation mechanisms in chromatin regulation
An important challenge in biology has been to understand how cell-type-specific expression programs are orchestrated through regulated access to chromatin. Knowledge of the interaction between noncoding RNAs (...
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Article
Open AccessHorizontal Gene Transfers in prokaryotes show differential preferences for metabolic and translational genes
Horizontal gene transfer (HGT) is an important process, which contributes in bacterial pathogenesis and drug resistance. A number of methods have been proposed for detection of horizontal gene transfer. One su...
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Article
Open AccessA novel method for prokaryotic promoter prediction based on DNA stability
In the post-genomic era, correct gene prediction has become one of the biggest challenges in genome annotation. Improved promoter prediction methods can be one step towards develo** more reliable ab initio gene...
