-
Article
Open AccessEfficient microwave-assisted extraction of salidroside from Rhodiola crenulata
A rapid microwave-assisted extraction (MAE) method was established to extract commercially important salidroside from dry plant materials of Rhodiola crenulata. Several parameters such as extraction time, solid/l...
-
Article
Open AccessFarnesol-induced hyperbranched morphology with short hyphae and bulbous tips of Coriolus versicolor
As the first fungal quorum sensing molecule, farnesol-induced morphological transition is usually studied in dimorphic fungi, but in basidiomycetes the morphological changes regulated by farnesol are rarely in...
-
Article
Lignin-Enhanced Laccase Production from Trametes versicolor
Laccase production requires a cost effective boost from host organisms to meet the needs for its numerous applications. Addition of 1.2 g L−1 lignin to media culture of Trametes versicolor improved laccase produc...
-
Article
Laccase Production from Trametes versicolor in Solid-State Fermentation of Steam-Exploded Pretreated Cornstalk
Laccase is an important enzyme used in removal of recalcitrant environmental wastes and industrial processes. The development of novel strategies for low-cost laccase production is needed to meet its demand. T...
-
Article
Scale-up laccase production from Trametes versicolor stimulated by vanillic acid
An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old myceli...
-
Article
Chitosan multiple addition enhances laccase production from Trametes versicolor
Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L−1 chitos...
-
Article
Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli
An efficient induction strategy that consisted of multiple additions of small doses of isopropyl-β-D-thiogalactopyranoside (IPTG) in the early cell growth phase was developed for enhancing Pfu DNA polymerase prod...
-
Article
Improved Biomass and Hydrocarbon Productivity of Botryococcus braunii by Periodic Ultrasound Stimulation
Ultrasonic treatment was firstly found to accelerate both biomass and hydrocarbon productivities of Botryococcus braunii algal cells cultured in the shake flasks. The most effective sonication strategy was to sub...
-
Article
Efficient Kinetic Resolution of (R,S)-2-Octanol Catalyzed by Magnetite-Immobilized Yarrowia lipolytica Lipase in Mixed Ionic Liquids
The resolution of (R,S)-2-octanol catalyzed by magnetite-immobilized Yarrowia lipolytica lipase in ionic liquids was investigated. The optimal activity and enantioselectivity of the lipase were obtained in a mixt...
-
Article
Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed
A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes vers...
-
Article
Low-temperature preincubation enhances survival and regeneration of cryopreserved Saussurea involucrata callus
Saussurea involucrata Kar. et Kir. is one of the most well-known Chinese medicinal plants, and it is utilized for a variety of medical conditions. Due to the overexploitation of this endangered s...
-
Article
Changes in endogenous hormones and oxidative burst as the biochemical basis for enhanced shoot organogenesis in cold-treated Saussurea involucrata explants
A low temperature treatment significantly improved shoot morphogenesis of Saussurea involucrata Kar. et Kir leaf explants. The biochemical mechanisms underlying the cold-induced shoot organogenesis were investiga...
-
Article
Immobilization of Trametes versicolor cultures for improving laccase production in bubble column reactor intensified by sonication
The mycelia of Trametes versicolor immobilized in alginate beads provided higher laccase production than that in pelleted form. An efficient ultrasonic treatment enhanced laccase production from the immobilized T...
-
Chapter
Hairy Root Culture: Bioreactor Design and Process Intensification
The cultivation of hairy roots for the production of secondary metabolites offers numerous advantages; hairy roots have a fast growth rate, are genetically stable, and are relatively simple to maintain in phyt...
-
Article
Gibberellic Acid Increases Secondary Metabolite Production in Echinacea purpurea Hairy Roots
Gibberellic acid (GA3) is reported to have diverse effects on hairy root cultures of many plant species; therefore, the effects of GA3 on the growth, secondary metabolite production (caffeic acid derivatives and ...
-
Article
Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir
An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explan...
-
Article
Heat Shock Treatment Improves Trametes versicolor Laccase Production
An efficient heat shock strategy has been developed to improve laccase production in submerged Trametes versicolor cultures. The optimized heat shock strategy consists of subjecting T. versicolor mycelial pellets...
-
Article
Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation
A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at...
-
Chapter
Biofuels in China: Opportunities and Challenges
With rapid economic development, energy consumption in China has tripled in the past 20 yr, exceeding 2.4 billion tons of standard coal in 2006. The search for new green energy as substitutes for the nonrenewa...
-
Article
Biofuels in China: opportunities and challenges
With rapid economic development, energy consumption in China has tripled in the past 20 yr, exceeding 2.4 billion tons of standard coal in 2006. The search for new green energy as substitutes for the nonrenewa...
