Abstract
An efficient heat shock strategy has been developed to improve laccase production in submerged Trametes versicolor cultures. The optimized heat shock strategy consists of subjecting T. versicolor mycelial pellets to three heat shock treatments at 45 °C for 45 min, starting at culture day 0, with a 24-h interval between treatments. Laccase production increased by more than 1.6-fold relative to the control in both flasks and a 5-L bioreactor because the expression of the laccase gene was enhanced by heat shock induction. The present work demonstrates that heat shock induction is a promising method because it both improves fungal laccase production and has a good potential in industrial application.
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References
Baldrian, P. (2006). Fungal laccases—Occurrence and properties. FEMS Microbiology Reviews, 30, 215–242.
Strong, P. J., & Claus, H. (2011). Laccase: A review of its past and its future in bioremediation. Critical Reviews in Environmental Science and Technology, 41, 373–434.
Osma, J. F., Moilanen, U., Toca-Herrera, J. L., & Rodriguez-Couto, S. (2011). Morphology and laccase production of white-rot fungi grown on wheat bran flakes under semi-solid-state fermentation conditions. FEMS Microbiology Letters, 318, 27–34.
Susana, R. C., & José, L. T. H. (2006). Industrial and biotechnological applications of laccases: A review. Biotechnology Advances, 24, 500–513.
Li, P., Wang, H. L., Liu, G. S., Li, X., & Yao, J. M. (2011). The effect of carbon source succession on laccase activity in the co-culture process of Ganoderma lucidum and a yeast. Enzyme and Microbial Technology, 48, 1–6.
Liu, Z. Y., Zhang, D. X., Hua, Z. Z., Li, J. H., Du, G. C., & Chen, J. (2010). Improvement of laccase production and its properties by low-energy ion implantation. Bioprocess and Biosystems Engineering, 33, 639–646.
Wu, Y. R., Luo, Z. H., Kwok-Kei Chow, R., & Vrijmoed, L. L. (2010). Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2. Bioresource Technology, 101, 9772–9777.
**g, D. B. (2010). Improving the simultaneous production of laccase and lignin peroxidase from Streptomyces lavendulae by medium optimization. Bioresource Technology, 101, 7592–7597.
Lopez, M., Loera, O., Guerrero-Olazaran, M., Viader-Salvado, J. M., Gallegos-Lopez, J. A., Fernandez, F. J., et al. (2010). Cell growth and Trametes versicolor laccase production in transformed Pichia pastoris cultured by solid-state or submerged fermentations. Journal of Chemical Technology and Biotechnology, 85, 435–440.
Fonseca, M. I., Shimizu, E., Zapata, P. D., & Villalba, L. L. (2010). Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina). Enzyme and Microbial Technology, 46, 534–539.
Umakoshi, H., Yoshimoto, M., Shimanouchi, T., Kuboi, R., & Komasawa, I. (1998). Model system for heat-induced translocation of β-galacosidase across phospholipids bilayer membrane. Biotechnology Progress, 14, 218–226.
Berovic, M., & Herga, M. (2007). Heat shock on Saccharomyces cerevisiae inoculum increases glycerol production in wine fermentation. Biotechnology Letters, 29, 891–894.
Himabindu, M., Potumarthi, R., & Jetty, A. (2007). Enhancement of gentamicin production by mutagenesis and non-nutritional stress conditions in Micromonospora echinospora. Process Biochemistry, 42, 1352–1356.
Babitha, S., Soccol, C. R., & Pandey, A. (2007). Effect of stress on growth, pigment production and morphology of Monascus sp. in solid cultures. Journal of Basic Microbiology, 47, 118–126.
Zhang, C., & Fevereiro, P. S. (2007). The effect of heat shock on paclitaxel production in Taxus yunnanensis cell suspension cultures—Role of abscisic acid pretreatment. Biotechnology and Bioengineering, 96, 506–514.
Chen, V. P., **e, H. Q., Chan, W. K., Leung, K. W., Choi, R. C., & Tsim, K. W. (2010). An induction effect of heat shock on the transcript of globular acetylcholinesterase in NG108-15 cells. Chemico-Biological Interactions, 187, 106–109.
Chou, C. P. (2007). Engineering cell physiology to enhance recombinant protein production in Escherichia coli. Applied Microbiology and Biotechnology, 76, 521–532.
Lee, K. T., Chen, S. C., Chiang, B. L., & Yamakawa, T. (2007). Heat-inducible production of beta-glucuronidase in tobacco hairy root cultures. Applied Microbiology and Biotechnology, 73, 1047–1053.
Wu, M. S., Pan, K. L., & Chou, C. P. (2007). Effect of heat-shock proteins for relieving physiological stress and enhancing the production of penicillin acylase in Escherichia coli. Biotechnology and Bioengineering, 96, 956–966.
Souza, J. V. B., Silva, E. S., Cavallazzi, J. R. P., & Sobrinho, A. D. (2010). Formulation of a liquid medium with wheat bran for the production of laccase by Trametes versicolor in an air-lift bioreactor. Journal of Food Agriculture and Environment, 8, 394–396.
Birhanli, E., & Yesilada, O. (2010). Enhanced production of laccase in repeated-batch cultures of Funalia trogii and Trametes versicolor. Biochemical Engineering Journal, 52, 33–37.
de Souza, E. S., Sampaio, I. D., Freire, A. K. D., da Silva, B. K. S., Sobrinho, A. D., Lima, A. M., et al. (2011). Production of Trametes versicolor laccase by solid state fermentation using a fixed-bed bioreactor. Journal of Food Agriculture and Environment, 9, 55–58.
Piscitelli, A., Giardina, P., Lettera, V., Pezzella, C., Sannia, G., & Faraco, V. (2011). Induction and transcriptional regulation of laccases in fungi. Current Genomics, 12, 104–112.
Hu, X. K., Zhao, X. H., & Hwang, H. M. (2007). Comparative study of immobilized Trametes versicolor laccase on nanoparticles and kaolinite. Chemosphere, 66, 1618–1626.
Jung, H., Xu, F., & Li, K. C. (2002). Purification and characterization of laccase from wood-degrading fungus Trichophyton rubrum LKY-7. Enzyme and Microbial Technology, 30, 161–168.
Collins, P. J., & Dobson, A. (1997). Regulation of laccase gene transcription in Trametes versicolo. Applied and Environmental Microbiology, 63, 3444–3450.
Tarkka, M. T., Vasara, R., Gorfer, M., & Raudaskoski, M. (2000). Molecular characterization of actin genes from homobasidiomycetes: Two different actin genes from Schizophyllum commune and Suillus bovinus. Gene, 251, 27–35.
Berovic, M., Pivec, A., Kosmerl, T., Wondra, M., & Celan, S. (2007). Influence of heat shock on glycerol production in alcohol fermentation. Journal of Bioscience and Bioengineering, 103, 135–139.
Galhaup, C., Goller, S., Peterbauer, C. K., Strauss, J., & Haltrich, D. (2002). Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions. Microbiology, 148, 2159–2169.
Okamoto, K., Ito, Y., Shigematsu, I., Yanagi, S. O., & Yanase, H. (2003). Cloning and characterization of a laccase gene from the white-rot basidiomycete Pleurotus ostreatus. Mycoscience, 44, 11–17.
Saloheimo, M., Niku-Paavola, M. L., & Knowles, J. K. (1991). Isolation and structural analysis of the laccase gene from the lignin-degrading fungus Phlebia radiata. Journal of General Microbiology, 137, 1537–1544.
Rancaño, G., Lorenzo, M., Molares, N., Rodríguez Couto, S., & Sanromán, Á. (2003). Production of laccase by Trametes versicolor in an airlift fermentor. Process Biochemistry, 39, 467–473.
Tavares, A. P. M., Coelho, M. A. Z., Agapito, M. S. M., Coutinho, J. A. P., & Xavier, A. M. R. B. (2006). Optimization and modeling of laccase production by Trametes versicolor in a bioreactor using statistical experimental design. Applied Biochemistry and Biotechnology, 134, 233–248.
Thiruchelvam, A. T., & Ramsay, J. A. (2007). Growth and laccase production kinetics of Trametes versicolor in a stirred tank reactor. Applied Microbiology and Biotechnology, 74, 547–554.
Acknowledgment
This work was financially supported by the National Natural Science Foundation of China (no. 21176241), the Bei**g Natural Science Foundation (no. 2102041), the National Key Technology Research and Development Program of China (no. 2012BAK25B01), and the Knowledge Innovation Program of the Chinese Academy of Sciences (nos. KSCX1-YW-11-D1 and KGCX2-YW-337). The authors thank Dr. Amanda R. Stiles (the Chinese Academy of Sciences Fellowships for Young International Scientists, no. 2011Y1GA01) for her assistance in editing the manuscript.
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Wang, F., Guo, C., Wei, T. et al. Heat Shock Treatment Improves Trametes versicolor Laccase Production. Appl Biochem Biotechnol 168, 256–265 (2012). https://doi.org/10.1007/s12010-012-9769-6
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DOI: https://doi.org/10.1007/s12010-012-9769-6