Abstract
Laccase production requires a cost effective boost from host organisms to meet the needs for its numerous applications. Addition of 1.2 g L−1 lignin to media culture of Trametes versicolor improved laccase production up to 1213.93 U L−1, which was 3.69-fold higher in comparison with that without lignin addition. The laccase production of 1009.49 U L−1 was achieved using the lignin-contained hydrolysate from steam-exploded corn stalk in practice. Elucidated mechanism of lignin-stimulated laccase production showed increased production of reactive oxygen species and the copy number of the laccase gene transcript in the treated mycelia. Together these results provide insight into some of the intrinsic metabolic processes involved in the up-regulation of laccase production in the presence of lignin. It also provides a low-cost strategy for the enzyme production from wastes and renewable biomass.
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Munk, L., Sitarz, A.K., Kalyani, D.C., Mikkelsen, J.D., Meyer, A.S.: Can laccases catalyze bond cleavage in lignin? Biotechnol. Adv. 33, 13–24 (2015)
Dwivedi, U.N., Singh, P., Pandey, V.P., Kumar, A.: Structure-function relationship among bacterial, fungal and plant laccases. J. Mol. Catal. B Enzym. 68, 117–128 (2011)
Rivera-Hoyos, C.M., Morales-Alvarez, E.D., Poutou-Pinales, R.A., Pedroza-Rodriguez, A.M., Rodriguez-Vazquez, R., Delgado-Boada, J.M.: Fungal laccases. Fungal Biol. Rev. 27, 67–82 (2013)
Osma, J.F., Toca-Herrera, J.L., Rodrıguez-Couto, S.: Cost analysis in laccase production. J. Environ. Manag. 92, 2907–2912 (2011)
Mann, J., Markham, J.L., Peiris, P., Spooner-Hart, R.N., Holford, P., Nair, N.G.: Use of olive mill wastewater as a suitable substrate for the production of laccase by Cerrena consors. Int. Biodeterior. Biodegrad. 99, 138–145 (2015)
Dhillon, G.S., Kaur, S., Brar, S.K., Verma, M.: Flocculation and haze removal from crude beer using in-house produced laccase from Trametes versicolor cultured on brewer’s spent grain. J. Agric. Food Chem. 60, 7895–7904 (2012)
Meyer, T., Edwards, E.A.: Anaerobic digestion of pulp and paper mill wastewater and sludge. Water Res. 65, 321–349 (2014)
Palmqvist, E., Hahn-Hagerdal, B.: Fermentation of lignocellulosic hydrolysates II: inhibitors and mechanisms of inhibition. Bioresour. Technol. 74, 25–33 (2000)
Wang, F., Guo, C., Wei, T., Zhang, T., Liu, C.Z.: Heat shock treatment improves Trametes versicolor laccase production. Appl. Biochem. Biotechnol. 168, 256–265 (2012)
Gerhardt, P., Murray, R.G.E., Wood, W.A., Krieg, N.R.: Methods for general and molecular bacteriology, ASM Washington DC, ISBE 1-55581-048-9, pp. 518 (1994)
Goering, H.K., Van-Soest, P.J.: Forage fiber analyses, apparatus, reagents, procedures and some applications. Washington, USDA. Agricultural handbook No. 379 (1970)
Piscitelli, A., Giardina, P., Lettera, V., Pezzella, C., Sannia, G., Faraco, V.: Induction and transcriptional regulation of laccases in fungi. Curr. Genomics 12, 104–112 (2011)
Wang, F., Hu, J., Guo, C., Liu, C.: Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer. Bioresour. Technol. 166, 602–605 (2014)
Marco-Urrea, E., Reddy, C.A.: Degradation of chloro-organic pollutants by white rot fungi. In: Singh, N.S. (ed.) Microbial Degradation of Xenobiotics: Environmental Science and Engineering, pp. 31–66. Springer, Heidelberg (2012)
Cajthaml, T., Svobodova, K.: Biodegradation of aromatic pollutanta by ligninolytic fungal strains. In: Singh, N.S. (ed.) Microbial Degradation of Xenobiotics: Environmental Science and Engineering, pp. 291–316. Springer, Heidelberg (2012)
Hofrichter, M., Ullrich, R., Pecyna, M.J., Liers, C., Lundell, T.: New and classic families of secreted fungal heme peroxidases. Appl. Microbiol. Biotechnol. 87, 871–897 (2010)
Haars, A., Huttermann, A.: Laccase induction in the white-rot fungus Heterobasidion annosum Fr. (Bref.). Arch. Microbiol. 134(4), 309–313 (1983)
Galhaup, C., Wagner, H., Hinterstoisser, B., Haltrich, D.: Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens. Enzyme Microb. Technol. 30, 529–536 (2002)
Adekunle, E.A., Wang, F., Hu, J., Ma, J., Guo, C., Zhuang, G., Liu, C.: Chitosan multiple addition enhances laccase production from Trametes versicolor. Bioprocess Biosyst. Eng. 38, 1973–1981 (2015)
Cap, M., Vachov, L., Palkova, Z.: Reactive oxygen species in the signalling and adaptation of multicellular microbial communities. Oxid. Med. Cell. Longev. (2012). doi:10.1155/2012/976753
Sigler, K., Chaloupka, J., Brozmanova, J., Stadler, N., Hofer, M.: Oxidative stress in microorganisms—I. microbial vs. higher cells—damage and defenses in relation to cell aging and Death. Folia Microbiol. 44(6), 587–624 (1999)
Coman, C., Mot, A.C., Gal, E., Parvu, M., Silaghi-Dumitrescu, R.: Laccase is upregulated via stress pathways in the phytopathogenic fungus Sclerotinia sclerotiorum. Fungal Biol. 117, 528–539 (2013)
Hiscox, J., Baldrian, P., Rogers, H.J., Boddy, L.: Changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus Trametes versicolor. Fungal Genent. Biol. 47, 562–571 (2010)
Soden, D.M., Dobson, A.D.: Differential regulation of laccase gene expression in Pleurotus sajor-caju. Microbiology 147, 1755–1763 (2001)
Eyre, C., Muftah, W., Hiscox, J., Hunt, J., Kille, P., Boddy, L., Rogers, H.J.: Microarray analysis of differential gene expression elicited in Trametes versicolor during interspecific mycelia interactions. Fungal Biol. 114, 646–660 (2010)
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This work was financially supported by the National Key Technology Research and Development Program of China (No. 2015BAK45B01), the National Natural Science Foundation of China (No. 21476242), and the National Basic Research Program (973 Program) of China (No. 2013CB733600).
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Adekunle, A.E., Guo, C. & Liu, CZ. Lignin-Enhanced Laccase Production from Trametes versicolor . Waste Biomass Valor 8, 1061–1066 (2017). https://doi.org/10.1007/s12649-016-9680-4
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DOI: https://doi.org/10.1007/s12649-016-9680-4