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Protocol
Saussurea medusa Cell Suspension Cultures for Flavonoid Production
Saussurea medusa Maxim. is a valuable traditional Chinese herb. The flavonoids are the main active pharmaceutical compounds in this medicinal plant species and have effective anti-tumor and anti-inflammation pro...
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Article
Enhancement of phenylethanoid glycosides biosynthesis in cell cultures of Cistanche deserticola by osmotic stress
The effect of osmotic stress on cell growth and phenylethanoid glycosides (PeGs) biosynthesis was investigated in cell suspension cultures of Cistanche deserticola Y. C. Ma, a desert medicinal plant grown in west...
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Article
Plant regeneration of Erigeron breviscapus (vant.) Hand. Mazz. and its chromatographic fingerprint analysis for quality control
An efficient micropropagation system for Erigeron breviscapus (vant.) Hand. Mazz., an important medicinal plant for heart disease, has been developed. Shoot organogenesis occurred from E. breviscapus leaf explant...
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Article
Echinacea biotechnology: Challenges and opportunities
Echinacea, better known as purple coneflower, has received a global attention because of its increasing medicinal value. There is enormous potential for the discovery of new medicinal com...
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Article
Light-enhanced caffeic acid derivatives biosynthesis in hairy root cultures of Echinacea purpurea
Light plays an important role in almost all plant developmental processes and provides the fundamental building blocks for growth, development, primary and secondary metabolism. The effects of light on growth ...
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Article
Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim
Cell growth, flavonoids biosynthesis and L-phenylalanine ammonia-lyase (PAL) activity were studied in callus cultures of Saussurea medusa Maxim. under different types of spectral radiance. After 21 days, red ligh...
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Article
In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.
An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on...
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Article
Plant regeneration from leaf explants of Rhodiola fastigiata
An efficient plant regeneration protocol for rapidly propagating Rhodiola fastigiata (Hk. f. et Thoms.) S.H.FU, a traditional Chinese medicinal plant, was developed. Shoot organogenesis occurred from the leaf exp...
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Protocol
Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts
A plant regeneration system from the isolated protoplasts of Echinacea purpurea L. using an alginate solid/liquid culture is described in the chapter. Viable protoplasts were isolated from 100 mg of young leaves ...
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Article
Comparison of Techniques for the Extraction of Flavonoids from Cultured Cells of Saussurea medusa Maxim
Room temperature, heat reflux, Soxhlet, ultrasound-assisted and microwave-assisted extractions were employed to extract flavonoids from dry cell cultures of Saussurea medusa Maxim. 24, 20, 6, 0.5 and 0.1 h exposu...
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Article
Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm conservation and elimination of heavy metal contamination
Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and...
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Article
Factors influencing artemisinin production from shoot cultures of Artemisia annua L.
Artemisinin, an anti-malarial drug isolated from the annual wormwood Artemisia annua L., has a marked activity against chloroquine-resistant and chloroquine-sensitive strains of Plasmodium falciparum, and is usef...
