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Immobilization of Trametes versicolor cultures for improving laccase production in bubble column reactor intensified by sonication

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The mycelia of Trametes versicolor immobilized in alginate beads provided higher laccase production than that in pelleted form. An efficient ultrasonic treatment enhanced laccase production from the immobilized T. versicolor cultures. The optimized treatment process consisted of exposing 36-h-old bead cultures to 7-min ultrasonic treatments twice with a 12-h interval using a fixed ultrasonic power and frequency (120 W, 40 kHz). Using the intensification strategy with sonication, laccase production increased by more than 2.1-fold greater than the untreated control in both flasks and bubble column reactors. The enhancement of laccase production by ultrasonic treatment is related to the improved mass transfer of nutrients and product between the liquid medium and the gel matrix. These results provide a basis for the large-scale and highly-efficient production of laccase using sonobioreactors.

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Abbreviations

C 0 :

Initial crystal violet concentration, μg/mL

C t :

Dynamic (after adsorption for t time) crystal violet concentration, μg/mL

m :

Weight of alginate beads, g

V :

Solution volume of crystal violet, mL

Q t :

Mass of crystal violet adsorbed at time t, μg/g wet gel

Q e :

Mass of crystal violet adsorbed at equilibrium, μg/g wet gel

K d :

Overall rate constant of adsorption, min−1

t :

Reaction time, min

TBA:

2-thiobarbituric acid

TBARS:

TBA-reactive substances

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Acknowledgments

This work was financially supported by the National Basic Research Program (973 Program) of China (No. 2013CB733600), the National Key Technology Research and Development Program of China (No. 2012BAK25B01), the Bei**g Natural Science Foundation (No. 2102041), the National High Technology Research and Development Program (863 Program) of China (No. 2012AA101803), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Nos. KSCX1-YW-11-D1 & KGCX2-YW-337). We acknowledge Dr. Amanda Stiles (the Chinese Academy of Sciences Fellowships for Young International Scientists, No. 2011Y1GA01) for the help in preparing the manuscript.

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  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Bei**g, 100190, People’s Republic of China

    Feng Wang, Chen Guo & Chun-Zhao Liu

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  1. Feng Wang
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  2. Chen Guo
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Correspondence to Chun-Zhao Liu.

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Wang, F., Guo, C. & Liu, CZ. Immobilization of Trametes versicolor cultures for improving laccase production in bubble column reactor intensified by sonication. J Ind Microbiol Biotechnol 40, 141–150 (2013). https://doi.org/10.1007/s10295-012-1214-x

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  • DOI: https://doi.org/10.1007/s10295-012-1214-x

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