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Changes in endogenous hormones and oxidative burst as the biochemical basis for enhanced shoot organogenesis in cold-treated Saussurea involucrata explants

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Abstract

A low temperature treatment significantly improved shoot morphogenesis of Saussurea involucrata Kar. et Kir leaf explants. The biochemical mechanisms underlying the cold-induced shoot organogenesis were investigated by measuring endogenous plant growth hormones, peroxide radicals, and superoxide dismutase (SOD) activity in the cold-treated leaf explants compared to controls. The ratio of zeatin (ZT) to indole-3-acetic acid (IAA) significantly increased in leaf explants subjected to a 5-day treatment at 4 °C compared to controls. The accumulation of O ·−2 also rapidly increased in response to cold treatment, and then decreased as SOD activity catalyzed the dismutation of O ·−2 to molecular oxygen and H2O2; this resulted in a significant increase in H2O2 concentrations in the cold-treated explants. We propose that a combination of the increased ZT/IAA ratio and H2O2 concentration is the basis for the enhanced shoot morphogenesis in response to cold treatment. These results provide a starting point for an improved understanding of the biochemical mechanisms underlying cold-induced shoot organogenesis of this unique plant species.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 21150110459), the Knowledge Innovation Program of the Chinese Academy of Sciences (No. YZ-06-03), and the Chinese Academy of Sciences Fellowship for Young International Scientists (No. 2011Y1GA01).

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Bei**g, 100190, People’s Republic of China

    Bin Guo, Amanda R. Stiles & Chun-Zhao Liu

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  1. Bin Guo
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  2. Amanda R. Stiles
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  3. Chun-Zhao Liu
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Correspondence to Chun-Zhao Liu.

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Communicated by K.-Y. Paek.

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Guo, B., Stiles, A.R. & Liu, CZ. Changes in endogenous hormones and oxidative burst as the biochemical basis for enhanced shoot organogenesis in cold-treated Saussurea involucrata explants. Acta Physiol Plant 35, 283–287 (2013). https://doi.org/10.1007/s11738-012-1052-5

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  • DOI: https://doi.org/10.1007/s11738-012-1052-5

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