138 Result(s)
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Protocol
Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro
Plant long non-coding RNAs (lncRNAs) have been implicated in many biological processes, including responses to abiotic stresses, yet their detailed functions and especially their modes of action are still unde...
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Protocol
Analysis of Raffinose Metabolism-Related Sugar Components in Seeds
Raffinose family oligosaccharides (RFOs) are synthesized from sucrose and subsequent addition of galactose moieties which was provided by galactinol. Galactinol is synthesized from UDP-galactose and myo-inositol....
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Protocol
Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea
Sclerotinia sclerotiorum and Botrytis cinerea are two closely related necrotrophic plant pathogenic fungi that have garnered significant attention due to their broad host range and persistent environmental presen...
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Protocol
T Cell Subsets and Immune Homeostasis
T cells are a heterogeneous group of cells that can be classified into different subtypes according to different classification methods. The body’s immune system has a highly complex and effective regulatory n...
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Protocol
ChIP and ChIRP Assays in Ferroptosis
Ferroptosis is characterized by the accumulation of lipid peroxidation driven by iron. As a regulated cell death, ferroptosis plays a critical role in various diseases and exhibits great therapeutic potentials...
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Protocol
Isolation and Identification of Plasma Extracellular Vesicles Protein Biomarkers
(EVs) have emerged as a valuable source for disease and an alternative drug delivery system due to their ability to carry cargo and target specific cells. Proper isolation, identification, and analytical st...
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Protocol
Genetic Encoding of a Fluorescent Noncanonical Amino Acid as a FRET Donor for the Analysis of Deubiquitinase Activities
The genetic code expansion technology enables the genetic encoding of fluorescent noncanonical amino acids (ncAAs) for site-specific fluorescent labeling of proteins. These co-translational and internal fluore...
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Protocol
Protein Expression with Biosynthesized Noncanonical Amino Acids
Natural proteins are normally made by 20 canonical amino acids. Genetic code expansion (GCE) enables incorporation of diverse chemically synthesized noncanonical amino acids (ncAAs) by orthogonal aminoacyl-tRN...
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Protocol
Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection
Early detection of solid tumors through a simple screening process, such as the proteomic analysis of biofluids, has the potential to significantly alter the management and outcomes of cancers. The application...
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Protocol
In Vitro Reconstitution of Pseudouridylation Catalyzed by Human Box H/ACA Ribonucleoprotein Particles
Pseudouridine (Ψ) is the most common chemical modification in RNA. In eukaryotes and archaea, pseudouridine synthases, mainly guided by box H/ACA snoRNAs, convert uridine to Ψ. Ψ stabilizes RNA structure and a...
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Protocol
Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout Using CRISPR-BETS
Cytosine base editors (CBEs) accurately modify target sites by mediating a C to T change (or a G to A change on the opposite strand). This allows us to install premature stop codons for gene knockout. However,...
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Protocol
Assessing the Activity of Transcription Factor FoxO1
The transcription factor FoxO1 (forkhead box O1) regulates genes that are involved in development, metabolism, cellular innovation, longevity, and stress responses. Assessment of FoxO1 activity is therefore cr...
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Protocol
Synthesis and Biochemical Evaluation of Monocarboxylic GRB2 SH2 Domain Inhibitors
This protocol discloses the synthesis of monocarboxylic inhibitors with a macrocyclic peptide scaffold to bind with the GRB2 SH2 domain and disrupt the protein–protein interactions (PPIs) between GRB2 and phos...
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Protocol
Bioinformatic Prediction of Bulked Oligonucleotide Probes for FISH Using Chorus2
Fluorescence in situ hybridization (FISH) provides great conveniences for detection and visualization of specific genomic segments. Oligonucleotide (Oligo)-based FISH further broadened the applications in plan...
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Protocol
The Value of Cell-Free Circulating DNA Profiling in Patients with Skin Diseases
Liquid biopsy, also known as fluid biopsy or fluid-phase biopsy, is the sampling and analysis of the blood, cerebrospinal fluid, saliva, pleural fluid, ascites, and urine. Compared with tissue biopsy, liquid b...
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Protocol
Targeting Transcription Factors in Cancer: From “Undruggable” to “Druggable”
Deregulation of transcription factors is critical to hallmarks of cancer. Genetic mutations, gene fusions, amplifications or deletions, epigenetic alternations, and aberrant post-transcriptional modification o...
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Protocol
The Interactome of Protein, DNA, and RNA
Proteins participate in many processes of the organism and are very important for maintaining the health of the organism. However, proteins cannot function independently in the body. They must interact with pr...
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Protocol
Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum
Clostridium cellulolyticum is a model mesophilic, cellulolytic bacterium, with the potential to produce biofuels from lignocellulose. However, the natural cellulose utilization efficiency is quite low and, theref...
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Protocol
Structural Variant Detection from Long-Read Sequencing Data with cuteSV
Structural Variation (SV) represents genomic rearrangements and is strongly associated with human health and disease. Recently, long-read sequencing technologies provide the opportunity to more comprehensive i...
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Protocol
Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling
The generation of cardiomyocytes (CMs) and endothelial cells (ECs) from human induced pluripotent stem cells (iPSCs) allows for precise modeling of cardiovascular disease using clinically relevant and patient-...
