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  1. No Access

    Protocol

    In Vitro Reconstitution of Pseudouridylation Catalyzed by Human Box H/ACA Ribonucleoprotein Particles

    Pseudouridine (Ψ) is the most common chemical modification in RNA. In eukaryotes and archaea, pseudouridine synthases, mainly guided by box H/ACA snoRNAs, convert uridine to Ψ. Ψ stabilizes RNA structure and a...

    Hironori Adachi, Jonathan L. Chen, Qiangzong Yin… in RNA-Protein Complexes and Interactions (2023)

  2. No Access

    Chapter

    Mechanisms and Clinical Applications of RNA Pseudouridylation

    Pseudouridylation is perhaps the most common epitranscriptomic modification among over 170 known chemical RNA modifications. Pseudouridine (Ψ) is highly conserved in various stable RNAs of all organisms. RNA p...

    Pedro Morais, Hironori Adachi, Jonathan L. Chen, Yi-Tao Yu in Epitranscriptomics (2021)

  3. No Access

    Protocol

    Detection and Quantification of Pseudouridine in RNA

    Pseudouridylation is the most abundant of all RNA modifications. Pseudouridylation is dynamic and widespread among many different types of RNAs in living organisms, thus drawing a lot of recent interest from t...

    Hironori Adachi, Meemanage D. DeZoysa, Yi-Tao Yu in Epitranscriptomics (2019)