SpringerLink
Log in

Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling

  • Protocol
  • First Online:
Induced Pluripotent Stem Cells and Human Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2549))

Abstract

The generation of cardiomyocytes (CMs) and endothelial cells (ECs) from human induced pluripotent stem cells (iPSCs) allows for precise modeling of cardiovascular disease using clinically relevant and patient-specific cells. Differentiation of human iPSCs into cardiomyocytes (iPSC-CMs) and endothelial cells (iPSC-ECs) is governed by small molecules that regulate the WNT signaling pathway. Here we outline the detailed steps to generate iPSC-CMs and iPSC-ECs through small molecule-mediated monolayer differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
EUR 44.95
Price includes VAT (Germany)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
EUR 99.99
Price includes VAT (Germany)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
EUR 128.39
Price includes VAT (Germany)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
EUR 181.89
Price includes VAT (Germany)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Protze SI, Lee JH, Keller GM (2019) Human pluripotent stem cell-derived cardiovascular cells: from developmental biology to therapeutic applications. Cell Stem Cell 25(3):311–327

    Article  CAS  Google Scholar 

  2. Zhao MT, Shao NY, Garg V (2020) Subtype-specific cardiomyocytes for precision medicine: where are we now? Stem Cells 38(7):822–833

    Article  Google Scholar 

  3. Kennedy CC, Brown EE, Abutaleb NO et al (2021) Development and application of endothelial cells derived from pluripotent stem cells in microphysiological systems models. Front Cardiovasc Med 8:625016

    Article  CAS  Google Scholar 

  4. Lin H, McBride KL, Garg V et al (2021) Decoding genetics of congenital heart disease using patient-derived induced pluripotent stem cells (iPSCs). Front Cell Dev Biol 9:630069

    Article  Google Scholar 

  5. Burridge PW, Matsa E, Shukla P et al (2014) Chemically defined generation of human cardiomyocytes. Nat Methods 11(8):855–860

    Article  CAS  Google Scholar 

  6. Zhao MT, Chen H, Liu Q et al (2017) Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs. Proc Natl Acad Sci U S A 114(52):E11111–E11120

    Article  CAS  Google Scholar 

  7. Ye S, Wan X, Su J et al (2021) Generation and expansion of human cardiomyocytes from patient peripheral blood mononuclear cells. J Vis Exp 168

    Google Scholar 

  8. Hu S, Zhao MT, Jahanbani F et al (2016) Effects of cellular origin on differentiation of human induced pluripotent stem cell-derived endothelial cells. JCI Insight 1(8):e85558

    Article  Google Scholar 

Download references

Acknowledgments

Research in Dr. Ming-Tao Zhao’s lab at Nationwide Children’s Hospital was supported by the American Heart Association (AHA) Career Development Award 18CDA34110293, NIH/NHLBI R01 grant HL155282-01, Additional Ventures Innovation Fund (AVIF), and Single Ventricle Research Fund (SVRF).

Author information

Authors and Affiliations

  1. Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, Columbus, OH, USA

    Arline Joachim, Shiqiao Ye & Ming-Tao Zhao

  2. The Heart Center, Nationwide Children’s Hospital, Columbus, OH, USA

    Arline Joachim, Shiqiao Ye & Ming-Tao Zhao

  3. Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA

    Ming-Tao Zhao

  4. Department of Physiology and Cell Biology, The Ohio State University College of Medicine, Columbus, OH, USA

    Ming-Tao Zhao

Authors
  1. Arline Joachim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shiqiao Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ming-Tao Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming-Tao Zhao .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Joachim, A., Ye, S., Zhao, MT. (2021). Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_427

Download citation

  • DOI: https://doi.org/10.1007/7651_2021_427

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2584-2

  • Online ISBN: 978-1-0716-2585-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation