1,003 Result(s)
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English
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Corrections to: Neurobiology: Methods and Protocols
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Correction to: ROS Signaling in Plants
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Correction to: New Technologies for Glutamate Interaction
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Correction to: Creation of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel Derived from a Size-Sorted Diploid Clone
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Correction to: Magnetic Resonance Spectroscopy (MRS) in Alzheimer’s Disease
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Correction to: Studying Bacteriophage Efficacy Using a Zebrafish Model
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Correction to: Estimating the Ca2+ Permeability of NMDA Receptors with Whole-Cell Patch-Clamp Electrophysiology
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Corrections to: Plastids
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Correction to: Tissue-Resident Macrophages: Methods and Protocols
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Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay
Magnetic tweezers are a force spectroscopy single-molecule technique. They enable the mechanical manipulation of biomolecules via the means of a magnetic particle under an attractive force applied by a magneti...
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Correction to: Xylem: Methods and Protocols
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Reagent Tracker™ Platform Verifies and Provides Audit Trails for the Error-Free Implementation of T-Cell ImmunoSpot® Assays
ELISPOT and FluoroSpot assays, collectively called ImmunoSpot assays, permit to reliable detection of rare antigen-specific T cells in freshly isolated cell material, such as peripheral blood mononuclear cells...
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Correction to: Immune Homeostasis: A Novel Example of Teamwork
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Ready-to-Record Cells for Kinetic Screening of VGICs
Voltage-gated ion channels (VGICs) are integral membrane proteins crucial for transmitting electrical signals in excitable cells. Understanding the kinetics of these ion channels requires conducting patch-clam...
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Use of Surface Plasmon Resonance Technique for Studies of Inter-domain Interactions in Ion Channels
Ion channels are transmembrane proteins essential for cellular functions and are important drug targets. Surface plasmon resonance (SPR) is a powerful technique for investigating protein–protein and protein–sm...
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Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans
Intracellular transport of organelles and biomolecules is vital for several cellular processes. Single-molecule fluorescence microscopy can illuminate molecular aspects of the dynamics of individual biomolecul...
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Fluorescence Microscopy of Nanochannel-Confined DNA
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and ...
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Artificial Intelligence-Based Counting Algorithm Enables Accurate and Detailed Analysis of the Broad Spectrum of Spot Morphologies Observed in Antigen-Specific B-Cell ELISPOT and FluoroSpot Assays
Antigen-specific B-cell ELISPOT and multicolor FluoroSpot assays, in which the membrane-bound antigen itself serves as the capture reagent for the antibodies that B cells secrete, inherently result in a broad ...
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Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA)
Spatially resolved mass spectrometry-based proteomics at single-cell resolution promises to provide insights into biological heterogeneity. We describe a protocol based on multiplexed data-independent acquisit...
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Quantifying ATP-Independent Nucleosome Chaperone Activity with Single-Molecule Methods
The dynamics of histone-DNA interactions govern chromosome organization and regulates the processes of transcription, replication, and repair. Accurate measurements of the energies and the kinetics of DNA bind...
