12 Result(s)

Protocol

Estimating the Ca²⁺ Block of NMDA Receptors with Single-Channel Electrophysiology

In vertebrate central neurons, NMDA receptors are glutamate- and glycine-gated ion channels that allow the passage of Na⁺ and Ca²⁺ ions into the cell when these neurotransmitters are simultaneously present. The p...

Gary J. Iacobucci, Gabriela K. Popescu in NMDA Receptors (2024)

Protocol

Estimating the Ca²⁺ Permeability of NMDA Receptors with Whole-Cell Patch-Clamp Electrophysiology

In the mammalian central nervous system (CNS), fast excitatory transmission relies primarily on the ionic fluxes generated by ionotropic glutamate receptors (iGluRs). Among iGluRs, NMDA receptors (NMDARs) are ...

Mae G. Weaver, Gabriela K. Popescu in NMDA Receptors (2024)

Article

Complex functional phenotypes of NMDA receptor disease variants

NMDA receptors have essential roles in the physiology of central excitatory synapses and their dysfunction causes severe neuropsychiatric symptoms. Recently, a series of genetic variants have been identified i...

Gary J. Iacobucci, Beiying Liu, Han Wen, Brittany Sincox… in Molecular Psychiatry (2022)

Article

NMDA receptors: linking physiological output to biophysical operation

Protocol

Extracting Rate Constants for NMDA Receptor Gating from One-Channel Current Recordings

Like all neurotransmitter-gated channels, in response to agonist binding, ionotropic glutamate receptors produce electrical signals whose amplitudes and durations reflect intramolecular transitions between non...

Kirstie A. Cummings, Gary J. Iacobucci… in Ionotropic Glutamate Receptor Technologies (2016)

Article

NMDA receptor activation requires remodelling of intersubunit contacts within ligand-binding heterodimers

Two classes of glutamate-activated channels mediate excitation at central synapses: N-methyl-D-aspartic acid (NMDA) receptors and non-NMDA receptors. Despite substantial structural homology, each class generates ...

William F. Borschel, Swetha E. Murthy, Eileen M. Kasperek… in Nature Communications (2011)

Article

Kinetic basis of partial agonism at NMDA receptors

The authors propose a mechanism of partial agonism, showing that partial agonists of both GluN1 and GluN2 NMDA receptor subunits have similar effects on the NMDA receptor activation reaction and they increase ...

Cassandra L Kussius, Gabriela K Popescu in Nature Neuroscience (2009)

Article

Distinct gating modes determine the biphasic relaxation of NMDA receptor currents

Following brief stimulation, macroscopic NMDA receptor currents decay with biphasic kinetics believed to reflect glutamate dissociation and receptor desensitization. The authors show that the fast and slow dec...

Wei Zhang, James R Howe, Gabriela K Popescu in Nature Neuroscience (2008)