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KRAS

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Andrew G. Stephen

    1. Frederick National Laboratory for Cancer Research, NCI RAS Initiative, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Dominic Esposito

    1. Frederick National Laboratory for Cancer Research, NCI RAS Initiative, Frederick, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2797)

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About this book

This volume details protocols ranging from high yield production metabolically labeled KRAS for NMR studies to approaches that quantify engagement of novel molecules that bind KRAS in live cells. Chapters focus on protein production and characterization, biochemical assays, cell-based assays, KRAS-membrane interactions, targeting KRAS, and cell models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, KRAS: Methods and Protocols aims to provide methods that will be instrumental in the development of future clinically approved KRAS therapeutics.

Keywords

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Progress in Targeting KRAS Directly

    • Dwight V. Nissley, Andrew G. Stephen, Ming Yi, Frank McCormick
    Pages 1-12

  3. The Abundance of KRAS and RAS Gene Mutations in Cancer

    • Edward C. Stites
    Pages 13-22

  4. Production of Isotopically Labeled KRAS4b

    • Troy Taylor, William Gillette
    Pages 23-34

  5. Nucleotide Exchange on RAS Proteins Using Hydrolysable and Non-hydrolysable Nucleotides

    • Timothy Waybright, Andrew G. Stephen
    Pages 35-46

  6. Crystallographic Studies of KRAS in Complex with Small Molecules and RAS-Binding Proteins

    • Albert H. Chan, Dhirendra K. Simanshu
    Pages 47-65

  7. Considerations Around Structure-Based Drug Discovery for KRAS Using DOCK

    • Mayukh Chakrabarti, Y. Stanley Tan, Trent E. Balius
    Pages 67-90

  8. Measurement of KRAS-GTPase Activity

    • Dana Rabara, Andrew G. Stephen
    Pages 91-102

  9. Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 to RAS Isoforms Using Surface Plasmon Resonance

    • Patrick Alexander, Andrew G. Stephen
    Pages 103-114

  10. 1D and 2D NMR for KRAS:Ligand Binding

    • Gabriel Cornilescu
    Pages 115-124

  11. Luminescence-Based Techniques for KRAS Thermal Stability Monitoring

    • Kari Kopra
    Pages 125-143

  12. MALDI-TOF Mass Spectrometry-Based Assay for Measuring Covalent Target Engagement of KRAS G12C Inhibitors

    • Marcin Dyba, John-Paul Denson, Anna E. Maciag
    Pages 145-157

  13. KRAS4b:RAF-1 Homogenous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Drug Discovery

    • Erik K. Larsen, Maria Abreu-Blanco, Dana Rabara, Andrew G. Stephen
    Pages 159-175

  14. Biophysical Characterization of RAS-SOS Complexes by Native Mass Spectrometry

    • Sangho Yun, Elena Scott, Arthur Laganowsky
    Pages 177-193

  15. Profiling Complex RAS-Effector Interactions Using NMR Spectroscopy

    • Regina Strakhova, Matthew J. Smith
    Pages 195-209

  16. Probing RAS Function Using Monobody and NanoBiT Technologies

    • Michael Whaby, Rakesh Sathish Nair, John P. O’Bryan
    Pages 211-225

  17. Analysis of the Guanine Nucleotide-Bound State of KRAS by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography

    • Makoto Araki, Kenji Kontani
    Pages 227-236

  18. Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy

    • Qingci Zhao, Ichio Shimada, Noritaka Nishida
    Pages 237-252

  19. Studying RAS Interactions in Live Cells with BRET

    • John Columbus, Thomas Turbyville
    Pages 253-260

  20. FLIM-FRET Protein-Protein Interaction Assay

    • Pedro Andrade Bonilla, Rebika Shrestha
    Pages 261-269
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Editors and Affiliations

  • Frederick National Laboratory for Cancer Research, NCI RAS Initiative, Frederick, USA

    Andrew G. Stephen, Dominic Esposito

Bibliographic Information

  • Book Title: KRAS

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew G. Stephen, Dominic Esposito

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3822-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Leios Biomedical Research, Inc. 2024

  • Hardcover ISBN: 978-1-0716-3821-7Published: 04 April 2024

  • Softcover ISBN: 978-1-0716-3824-8Due: 05 July 2024

  • eBook ISBN: 978-1-0716-3822-4Published: 03 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 365

  • Number of Illustrations: 14 b/w illustrations, 69 illustrations in colour

  • Topics: Cancer Research, Chemistry/Food Science, general

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