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  1. Protocol

    Reagent Tracker Platform Verifies and Provides Audit Trails for the Error-Free Implementation of T-Cell ImmunoSpot® Assays

    ELISPOT and FluoroSpot assays, collectively called ImmunoSpot assays, permit to reliable detection of rare antigen-specific T cells in freshly isolated cell material, such as peripheral blood mononuclear cells...

    Alexander A. Lehmann, Diana R. Roen, Zoltán Megyesi in Handbook of ELISPOT (2024)

  2. No Access

    Protocol

    Unbiased, High-Throughput Identification of T Cell Epitopes by ELISPOT

    Recent systematic immune monitoring efforts suggest that, in humans, epitope recognition by T cells is far more complex than has been assumed based on minimalistic murine models. The increased complexity is du...

    Paul V. Lehmann, Diana R. Roen, Alexander A. Lehmann in Computational Vaccine Design (2023)

  3. Article

    Open Access

    Prediction of B cell epitopes in proteins using a novel sequence similarity-based method

    Prediction of B cell epitopes that can replace the antigen for antibody production and detection is of great interest for research and the biotech industry. Here, we developed a novel BLAST-based method to pre...

    Alvaro Ras-Carmona, Alexander A. Lehmann, Paul V. Lehmann in Scientific Reports (2022)