Single Molecule Analysis
Methods and Protocols
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Protocol
Surface Functionalization, Nucleic Acid Tether Characterization, and Force Calibration for a Magnetic Tweezers Assay
Magnetic tweezers are a force spectroscopy single-molecule technique. They enable the mechanical manipulation of biomolecules via the means of a magnetic particle under an attractive force applied by a magneti...
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Protocol
An Introduction to Magnetic Tweezers
Magnetic tweezers are a single-molecule force and torque spectroscopy technique that enable the mechanical interrogation in vitro of biomolecules, such as nucleic acids and proteins. They use a magnetic field ...
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Book
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Chapter
A Single-Molecule View on Cellular and Viral RNA Synthesis
RNA synthesis is at the heart of gene expression, the RNA products serving in cellular organisms as protein blueprints, structural/functional components of molecular machines or regulators of gene expression, ...
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Article
Open AccessAuthor Correction: Correction-free force calibration for magnetic tweezers experiments
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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Article
Open AccessCorrection-free force calibration for magnetic tweezers experiments
Magnetic tweezers are a powerful technique to perform high-throughput and high-resolution force spectroscopy experiments at the single-molecule level. The camera-based detection of magnetic tweezers enables th...
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Article
Open AccessPausing controls branching between productive and non-productive pathways during initial transcription in bacteria
Transcription in bacteria is controlled by multiple molecular mechanisms that precisely regulate gene expression. It has been recently shown that initial RNA synthesis by the bacterial RNA polymerase (RNAP) is...
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Article
Strand separation establishes a sustained lock at the Tus–Ter replication fork barrier
Tus protein bound to Ter sites on circular bacterial chromosomes provides a way to avoid random crashes of opposing replication forks. DNA-unzip** experiments show that the Tus–Ter–induced lock during unzip**...
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Article
Studying genomic processes at the single-molecule level: introducing the tools and applications
We present key examples of the use of single-molecule approaches to study transcription, translation, splicing and replication.
