Neurobiology
Methods and Protocols
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Open AccessThe Role of PLAG1 in Mouse Brain Development and Neurogenesis
The pleomorphic adenoma gene 1 (Plag1) is a transcription factor involved in the regulation of growth and cellular proliferation. Here, we report the spatial distribution and functional implications of PLAG1 expr...
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Protocol
Corrections to: Neurobiology: Methods and Protocols
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Protocol
Neural Stem/Progenitor Cell (NSPC) Extraction and Culture
Neural stem–progenitor cells (NSPCs) are multipotent, self-renewing cells that generate radial glial cells (RGC). RGCs then give rise to neurons and glia during neural development. Here, we describe the proces...
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In Situ Hybridization to Characterize Neurulation and Midbrain–Hindbrain Boundary Formation in Zebrafish
Whole-mount in situ hybridization is able to harness the inherent advantages of zebrafish as a model organism for developmental biology, particularly when visualizing the formation of the neural tube, specific...
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Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue
Cryopreservation and immunohistochemistry offer a comprehensive, robust, and simple methodology to investigate neural patterning and cellular function. Rapid freezing of the whole brain allows excellent preser...
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Methodology for Free-Floating Organ Culture of Mid-gestation Maxillary Primordial Tissue
Craniofacial defects, such as cleft palate, are prevalent congenital malformations that present an interesting research challenge due to the complex and multifactorial nature of their etiology. In vitro modeli...
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Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development
Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zeb...
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Open AccessLoss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis
Identifying soluble factors that influence epidermal integrity is critical for the development of preventative and therapeutic strategies for disorders such as ichthyosis, psoriasis, dermatitis and epidermal c...
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Stage-dependent therapeutic efficacy in PI3K/mTOR-driven squamous cell carcinoma of the skin
Cutaneous squamous cell carcinoma (SCC) is a recurrent cancer that is prevalent in predisposed subjects such as immunosuppressed patients and patients being treated for other malignancies. Model systems to tri...
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Open AccessMis-expression of grainyhead-like transcription factors in zebrafish leads to defects in envelo** layer (EVL) integrity, cellular morphogenesis and axial extension
The grainyhead-like (grhl) transcription factors play crucial roles in craniofacial development, epithelial morphogenesis, neural tube closure, and dorso-ventral patterning. By utilising the zebrafish to differen...
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Open AccessMice lacking the conserved transcription factor Grainyhead-like 3 (Grhl3) display increased apposition of the frontal and parietal bones during embryonic development
Increased apposition of the frontal and parietal bones of the skull during embryogenesis may be a risk factor for the subsequent development of premature skull fusion, or craniosynostosis. Human craniosynostos...
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Novel mechanisms that pattern and shape the midbrain-hindbrain boundary
The midbrain-hindbrain boundary (MHB) is a highly conserved vertebrate signalling centre, acting to pattern and establish neural identities within the brain. While the core signalling pathways regulating MHB f...
