9 Result(s)
within
Martin Krönke
Immunology
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Article
Open AccessCHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
The BH3-only protein NOXA is a regulator of mitochondrial apoptosis by specifically antagonizing the anti-apoptotic protein MCL-1. Here we show that the E3 ubiquitin ligase CHIP controls NOXA stability after D...
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Article
Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes
Granules containing perforin and granzymes are secreted from cytotoxic T lymphocytes. Krönke and co-workers find that acid sphingomyelase is needed for granule shrinkage just before exocytosis in this process.
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Article
Interleukin-4 impairs granzyme-mediated cytotoxicity of Simian virus 40 large tumor antigen-specific CTL in BALB/c mice
In this report we analyzed the impact of interleukin-4 (IL-4) on tumor-associated simian virus 40 (SV40) large T-antigen (TAg)-specific CD8+ cytotoxic T cells during rejection of syngeneic SV40 transformed mKSA t...
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Article
Expression of lysostaphin in HeLa cells protects from host cell killing by intracellular Staphylococcus aureus
The Staphylococcus aureus-specific cell wall endopeptidase lysostaphin was used as a model for an intracellular acting bactericidal antibiotic. HeLa cells were transfected with an expression vector directing the ...
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Chapter
Ceramide as an Activator Lipid of Cathepsin D
We have identified the aspartic protease cathepsin D as a novel intracellular target protein for the lipid second messenger ceramide. Ceramide specifically binds to and induces CTSD proteolytic activity. A-SMa...
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Chapter
Molecular Cloning and Expression of cDNAS Encoding the Human Interleukin-2 Receptor
We have identified cDNAs encoding the human interleu- kin-2 (IL-2) receptor from a cDNA library constructed from HUT-102B2 cell mRNA, and have expressed them in eukaryotic cells. Based on the deduced amino aci...
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Chapter
Frequency-Analysis of Precursors of Cytotoxic T Lymphocytes in Radiation Chimeras: Enumeration of Antigenspecific CTL-P Restricted to Thymic MHC- and Bone Marrow-MHC-Determinants
The mechanisms controlling the acquisition of T cell restriction specificity and immunocompetence are, despite of numerous investigations, not well understood. From studies of the CTL-immune responsiveness in ...
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Chapter
Functional Analysis of Alloreactive Helper T Cells Involved in the Induction of Cytolytic T Cell Responses In Vitro
When T-responder cells are sensitized in vitro to foreign antigen presented on syngeneic cells or towards allogeneic stimulator cells, a proliferative response is initiated in which antigen specific...
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Article
Linkage of C4 and C4 deficiency to BF and GPLA
The C4, Bf, and GPLA phenotypes of homo- and heterozygous C4-deficient guinea pigs were studied. The electrophoretic patterns suggest that the deficiency in circulating C4 results from an impaired structural g...
