-
Article
Open AccessThe importance of murine phospho-MLKL-S345 in situ detection for necroptosis assessment in vivo
Necroptosis is a caspase-independent modality of cell death implicated in many inflammatory pathologies. The execution of this pathway requires the formation of a cytosolic platform that comprises RIPK1 and RI...
-
Article
Granzyme serine proteases in inflammation and rheumatic diseases
Granzymes (granule-secreted enzymes) are a family of serine proteases that have been viewed as redundant cytotoxic enzymes since their discovery more than 30 years ago. Predominantly produced by cytotoxic lymp...
-
Article
Open AccessIdentification of FasL as a crucial host factor driving COVID-19 pathology and lethality
The dysregulated immune response and inflammation resulting in severe COVID-19 are still incompletely understood. Having recently determined that aberrant death-ligand-induced cell death can cause lethal infla...
-
Article
Open AccessMultiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides
Self-assembling peptides (SAPs) have gained significant attention in biomedicine because of their unique properties and ability to undergo molecular self-assembly driven by non-covalent interactions. By manipu...
-
Article
Open AccessSeroprevalence of anti-SARS-CoV-2 antibodies in household domestic ferrets (Mustela putorius furo) in Spain, 2019–2023
SARS-CoV-2 is the causal agent of Coronavirus Disease 2019 (COVID-19) in humans that emerged in late 2019. This virus is able to infect humans and different animal species. Among pets, cats and ferrets are mor...
-
Article
Open AccessUnravelling the cellular response to the SARS-COV-2 vaccine in inflammatory bowel disease patients on biologic drugs
Suboptimal vaccine response is a significant concern in patients with Inflammatory Bowel Disease (IBD) receiving biologic drugs. This single-center observational study involved 754 patients with IBD. In Phase ...
-
Article
Open AccessThe dynamics of neutralizing antibodies against SARS-CoV-2 in cats naturally exposed to virus reveals an increase in antibody activity after re-infection
Severe Acute Respiratory Syndrome Coronavirus 2 is the causative agent of Coronavirus Disease 2019 in humans. To date, little is known about the persistence of antibodies against SARS-CoV-2 in animals under na...
-
Article
Open AccessIntravenous administration of BCG in mice promotes natural killer and T cell-mediated antitumor immunity in the lung
Intravesical administration of Bacillus Calmette-Guérin (BCG) was one of the first FDA-approved immunotherapies and remains a standard treatment for bladder cancer. Previous studies have demonstrated that intr...
-
Article
Open AccessSerum lipid mediator profiles in COVID-19 patients and lung disease severity: a pilot study
Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 infection is highly heterogeneous, ranging from asymptomatic to severe and fatal cases. COVID-19 has been characterized by an increase of serum pro-infl...
-
Article
Open AccessIdentification of an ASC oligomerization inhibitor for the treatment of inflammatory diseases
The ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD)) protein is an scaffold component of different inflammasomes, intracellular multiprotein platforms of the innate ...
-
Protocol
Mouse Model of Colitis-Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells
Colorectal cancer (CRC) is the third most common malignancy worldwide presenting high mortality due to low treatment efficacy. Existing evidence indicates that inflammatory bowel disease (IBD) is associated wi...
-
Article
Antigen-specific primed cytotoxic T cells eliminate tumour cells in vivo and prevent tumour development, regardless of the presence of anti-apoptotic mutations conferring drug resistance
Cytotoxic CD8+ T (Tc) cells are the main executors of transformed and cancer cells during cancer immunotherapy. The latest clinical results evidence a high efficacy of novel immunotherapy agents that modulate Tc ...
-
Article
Open AccessMultiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo
The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cell...
-
Article
Open AccessImmunoproteomic identification and characterization of Ni2+-regulated proteins implicates Ni2+ in the induction of monocyte cell death
Nickel allergy is the most common cause of allergic reactions worldwide, with cutaneous and systemic effects potentially affecting multiple organs. Monocytes are precursors of not only macrophages but also den...
-
Article
Open AccessDevelopment and characterization of a microfluidic model of the tumour microenvironment
The physical microenvironment of tumours is characterized by heterotypic cell interactions and physiological gradients of nutrients, waste products and oxygen. This tumour microenvironment has a major impact o...
-
Article
Clinical validity of bis(methylthio)gliotoxin for the diagnosis of invasive aspergillosis
Early and accurate diagnosis of invasive aspergillosis (IA) is one of the most critical steps needed to efficiently treat the infection and reduce the high mortality rates that can occur. We have previously fo...
-
Article
Second meeting of the Spanish Immunotherapy Group GEIT (Grupo Español de Inmunoterapia), January 16, 2015, in Zaragoza, Spain
-
Article
In Vitro Fungicidal Photodynamic Effect of Hypericin on Trichophyton spp
Hypericin is a natural photosensitizer used in photodynamic therapy (PDT), which has shown in vitro antifungal effect against Candida spp. The aim of this study was to evaluate the in vitro fungicidal effect of h...
-
Article
Open AccessEssential complicity of perforin-granzyme and FAS-L mechanisms to achieve tumor rejection following treatment with anti-CD137 mAb
Treatment with agonist anti-CD137 (4-1BB) immunostimulatory monoclonal antibodies elicits complete tumor regressions in a number of transplanted hematological and solid malignancies in mice. Rejection is mainl...
-
Article
Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes
Granules containing perforin and granzymes are secreted from cytotoxic T lymphocytes. Krönke and co-workers find that acid sphingomyelase is needed for granule shrinkage just before exocytosis in this process.
