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Article
Open AccessThe complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest
The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a ...
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Chapter
Noncoding RNA Genes Transcribed by RNA Polymerase III in Yarrowia lipolytica
In the hemiascomycetous yeast Yarrowia lipolytica, the Pol III-transcribed ncRNAs display a number of unusual and remarkable features with respect to other Hemiascomycetes. With 510 bona fide genes, the genome of...
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Chapter
Comparative Genomics of Yarrowia lipolytica
Well known for its potential for industrial applications detailed in other chapters of this book, Yarrowia lipolytica occupies an interesting position among hemiascomycetous yeasts due to its basal position in th...
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Article
An overview of lipid metabolism in yeasts and its impact on biotechnological processes
High energy prices, depletion of crude oil supplies, and price imbalance created by the increasing demand of plant oils or animal fat for biodiesel and specific lipid derivatives such as lubricants, adhesives,...
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Article
Open AccessDetection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts
Hemiascomycetous yeasts have intron-poor genomes with very few cases of alternative splicing. Most of the reported examples result from intron retention in Saccharomyces cerevisiae and some have been shown to be ...
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Chapter
Applications of the Non-Conventional Yeast Yarrowia lipolytica
The yeast Yarrowia lipolytica is often found associated to proteinaceous or hydrophobic substrates such as alkanes or lipids. To assimilate these hydropho-bic substrates, Y. lipolytica has developed an adaptative...
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Article
Functional Analysis of the MATB Mating-Type Idiomorph of the Dimorphic Fungus Yarrowia lipolytica
The whole MATA cassette from Yarrowia lipolytica, a dimorphic fungus, was replaced by the URA3 gene through a double homologous recombination. This MAT-less strain lost its mate capacity with A or B Y. lipolytica
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Article
Genome evolution in yeasts
Identifying the mechanisms of eukaryotic genome evolution by comparative genomics is often complicated by the multiplicity of events that have taken place throughout the history of individual lineages, leaving...
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Article
Chromosomal polymorphism in the yeast species Debaryomyces hansenii
Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...
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Article
Chromosomal polymorphism in the yeast species Debaryomyces hansenii
Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic ...
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Chapter
Functional genetics of Yarrowia lipolytica
The yeast Yarrowia lipolytica has been used for industrial bioconversions since the late 1960’s, mainly by the food and chemical industries. The most important process is presently the production of citric acid f...
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Article
Open AccessUse of specific DNA probes for the rapid characterization of yeasts isolated from complex biotopes
We describe a rapid method developed to identify yeast species commonly found during cheese ripening. It is based on the isolation of species-specific sequences and their hybridization with coarse preparations...
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Article
Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene
Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse po...
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Chapter
Yarrowia lipolytica
Interest in Candida lipolytica (Harrison) Diddens et Lodder 1942 initially arose from its rather uncommon physiological characteristics. Strains of this species were more often isolated from lipid- or protein-con...
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Article
Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue
Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a hi...
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Article
Multiple-copy integration in the yeast Yarrowia lipolytica
Using an EcoRI-BglII fragment of the G unit of the rDNA of Y. lipolytica and a set of 11 deletions in the URA3 promoter, we have constructed several plasmids to test gene amplification in the rDNA. These plasmids...
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Article
LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast
In the yeastYarrowia lipolytica, theLYC1 locus controls the first step of the lysine degradation pathway which is catalyzed by lysine N-6-acetyl transferase (LAT). This gene was cloned by complementation of thely...
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Article
Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes
Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains s...
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Article
Hyperthermostable Variants of a Highly Thermostable Alpha-Amylase
Genetic screening at temperatures between 70–80°C far exceeds the range of growth of most bacteria, and is not applicable to isolate easily thermostable protein variants. We describe a temperature shift protoc...
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Article
Cloning of the mating-type gene MATA of the yeast Yarrowia lipolytica
The mating type gene MA TA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for...
