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Article
The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstra...
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Article
Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker
Few selective markers are available for the transformation of the industrial yeast Yarrowia lipolytica, and those that are require the use of specialized hosts (e.g., auxotrophs, antibiotic sensitive). To enable ...
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Article
Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by target integration
A Yarrowia lipolytica yeast gene bank has been constructed in E. coli in an integrative plasmid vector containing the homologous LEU2 gene, and used to transform a leu2 lys5 yeast strain. The LYS5 gene encoding s...
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Article
LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica
The nucleotide sequence of a 968 by DNA fragment spanning the promoter and the 5′ upstream sequence of the LEU2 coding sequence of the yeast Yarrowia lipolytica has been determined. A LEU2:lacZ gene fusion has be...
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Article
Replication and recombination of 2-µm DNA in Schizosaccharomyces pombe
Any one of the inverted sequences present on the 2-µm DNA from Saccharomyces cerevisiae can promote replication of chimeric plasmids in Schizosaccharomyces pombe. When however a complete 2-µm molecule is present ...
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Article
Evidence for mutations in the structural gene for homocitrate synthase in Saccharomycopsis lipolytica
Eight strains devoid of homocitrate synthase activity were found among lysine requiring mutants of the yeast Saccharomycopsis lipolytica. Genetic analysis of these strains showed that they were all affected at th...
