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Article
Open AccessIn-depth analysis of erythrose reductase homologs in Yarrowia lipolytica
The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-ery...
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Article
Open AccessA 37-amino acid loop in the Yarrowia lipolytica hexokinase impacts its activity and affinity and modulates gene expression
The oleaginous yeast Yarrowia lipolytica is a potent cell factory as it is able to use a wide variety of carbon sources to convert waste materials into value-added products. Nonetheless, there are still gaps in o...
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Article
Open AccessIdentification of telomerase RNAs in species of the Yarrowia clade provides insights into the co-evolution of telomerase, telomeric repeats and telomere-binding proteins
Telomeric repeats in fungi of the subphylum Saccharomycotina exhibit great inter- and intra-species variability in length and sequence. Such variations challenged telomeric DNA-binding proteins that co-evolved...
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Article
Open AccessBlastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars
In the context of sustainable development, yeast are one class of microorganisms foreseen for the production of oil from diverse renewable feedstocks, in particular those that do not compete with the food supp...
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Article
Open AccessExon junction complex components Y14 and Mago still play a role in budding yeast
Since their divergence from Pezizomycotina, the mRNA metabolism of budding yeasts have undergone regressive evolution. With the dramatic loss of introns, a number of quality control mechanisms have been simpli...
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Article
Open AccessEUF1 – a newly identified gene involved in erythritol utilization in Yarrowia lipolytica
The gene YALI0F01562g was identified as an important factor involved in erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative an...
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Protocol
Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data
The number of fully sequenced genomes of yeasts is dramatically increasing but both structural and functional annotation quality are usually neglected, as most frequently based on automatic annotation transfer...
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Article
Open AccessAwakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials is of major interest for sustainable food and energy. Galactose is a monosaccharide found in galactomannans, hemicelluloses,...
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Article
Open AccessThe complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest
The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a ...
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Article
Open AccessComparative genomics of emerging pathogens in the Candida glabrata clade
Candida glabrata follows C. albicans as the second or third most prevalent cause of candidemia worldwide. These two pathogenic yeasts are distantly related, C. glabrata being part of the Nakaseomyces, a group mor...
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Chapter
Noncoding RNA Genes Transcribed by RNA Polymerase III in Yarrowia lipolytica
In the hemiascomycetous yeast Yarrowia lipolytica, the Pol III-transcribed ncRNAs display a number of unusual and remarkable features with respect to other Hemiascomycetes. With 510 bona fide genes, the genome of...
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Chapter
Comparative Genomics of Yarrowia lipolytica
Well known for its potential for industrial applications detailed in other chapters of this book, Yarrowia lipolytica occupies an interesting position among hemiascomycetous yeasts due to its basal position in th...
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Article
Engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast Yarrowia lipolytica by modifying the ß-oxidation multifunctional protein
Recombinant strains of the oleaginous yeast Yarrowia lipolytica expressing the PHA synthase gene (PhaC) from Pseudomonas aeruginosa in the peroxisome were found able to produce polyhydroxyalkanoates (PHA). PHA pr...
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Article
Open AccessDetection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts
Hemiascomycetous yeasts have intron-poor genomes with very few cases of alternative splicing. Most of the reported examples result from intron retention in Saccharomyces cerevisiae and some have been shown to be ...
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Article
Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen
Yarrowia lipolytica is a strictly aerobic fungus, which differs from the extensively studied model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe with respect to its physiology, genetics and dimorp...
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Article
Genome evolution in yeasts
Identifying the mechanisms of eukaryotic genome evolution by comparative genomics is often complicated by the multiplicity of events that have taken place throughout the history of individual lineages, leaving...
