Yarrowia lipolytica
Biotechnological Applications
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The influence of oxygen limitation for the production of succinic acid with recombinant strains of Yarrowia lipolytica
The yeast Yarrowia lipolytica is able to produce high amounts of several organic acids such as pyruvic, citric, isocitric, alpha-ketoglutaric, and succinic acid. Here we report on the influence of the reduced act...
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Engineering the α-ketoglutarate overproduction from raw glycerol by overexpression of the genes encoding NADP+-dependent isocitrate dehydrogenase and pyruvate carboxylase in Yarrowia lipolytica
To establish and develop a biotechnological process of α-ketoglutaric acid (KGA) production by Yarrowia lipolytica, it is necessary to increase the KGA productivity and to reduce the amounts of by-products, e.g. ...
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Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining
The ascomycetous yeast Yarrowia lipolytica has been established as model system for studies of several research topics as well as for biotechnological processes in the last two decades. However, frequency of hete...
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Book
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Book
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Chapter
Production of Organic Acids by Yarrowia lipolytica
Wild-type strains of Yarrowia lipolytica secrete several organic acids like citric acid, isocitric acid, α-ketoglutaric acid, fumaric acid, malic acid or pyruvic acid, under certain circumstances from different c...
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Transposable Elements and Their Activities in Y. lipolytica
Y. lipolytica harbors an unusually diverse set of transposable elements among Saccharomycotina yeasts. Among them, members of both the families of transposons, retrotransposons as well as DNA trans...
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The Gpr1/Fun34/YaaH Protein Family in the Nonconventional Yeast Yarrowia lipolytica and the Conventional Yeast Saccharomyces cerevisiae
The sequencing of the genomes of several organisms was the first step in understanding genome organisation, gene function and of course life itself. Until now very much information could be gained by searching...
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Article
Variation of the by-product spectrum during α-ketoglutaric acid production from raw glycerol by overexpression of fumarase and pyruvate carboxylase genes in Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric, isocitric, pyruvic (PA), and α-ketoglutaric (KGA) acids, triggered by growth limitation and excess of carbon source. This...
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Microbiologically Produced Carboxylic Acids Used as Building Blocks in Organic Synthesis
Oxo- and hydroxy-carboxylic acids are of special interest in organic synthesis. However, their introduction by chemical reactions tends to be troublesome especially with regard to stereoselectivity. We describ...
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Overproduction and secretion of α-ketoglutaric acid by microorganisms
This mini-review presents a summary of research results of biotechnological production of alpha-ketoglutaric acid (KGA) by bacteria and yeasts. KGA is of particular industrial interest due to its broad applica...
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Overexpression of alpha-ketoglutarate dehydrogenase in Yarrowia lipolytica and its effect on production of organic acids
The yeast Yarrowia lipolytica is one of the most intensively studied “non-conventional” yeast species. Its ability to secrete various organic acids, like pyruvic (PA), citric, isocitric, and alpha-ketoglutaric (K...
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Article
Aconitase overexpression changes the product ratio of citric acid production by Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric acid (CA) and isocitric acid (ICA) under an excess of carbon source and several conditions of growth limitation. Depending...
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Article
Overexpression of the ICL1 gene changes the product ratio of citric acid production by Yarrowia lipolytica
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric (CA) and isocitric (ICA) acids, triggered by growth limitation caused by different factors and an excess of carbon source....
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Article
Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica
The yeast Yarrowia lipolytica is able to secrete high amounts of several organic acids under conditions of growth limitation and carbon source excess. Here we report the production of citric acid (CA) in a fed-ba...
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Chapter
Mating, Sporulation and Random Spore Selection by Nystatin in Yarrowia lipolytica
To study the life cycle of the yeast Yarrowia lipolytica by observation of zygote formation and sporulation followed by spore analysis.
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Detection of Biosynthesis and Degradation of Peroxisomes by Different Carbon Sources with a Combined Assay Using a β-Galactosidase-GFP-SKL Chimaeric Protein for Labeling of Peroxisomes and the Peroxisomal Isocitrate Lyase as Control
To study the induction and repression of biogenesis of peroxisomes on different carbon sources.
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Detection and Activation of the Ylt1 Retrotransposon in the Yeast Yarrowia lipolytica
To study the presence, copy number and induction of transposition of the Yltl retrotransposon in the yeast Yarrowia lipolytica.
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Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media
To express a mutated gene, causing sensitivity to acetic acid, and to study the effects on growth of transformants on acetic acid/acetate containing media.
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Induction of Citric Acid/lsocitric Acid and α-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica
To induce the overproduction of citric and isocitric acids at different proportions, as well as a-ketoglutaric acid, in Y. lipolytica by changing the cultivation conditions.
