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Article
Open AccessNew recognition specificity in a plant immune receptor by molecular engineering of its integrated domain
Plant nucleotide-binding and leucine-rich repeat domain proteins (NLRs) are immune sensors that recognize pathogen effectors. Here, we show that molecular engineering of the integrated decoy domain (ID) of an ...
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Article
NMR chemical shift assignment of a constitutively active fragment of the antitermination protein LicT
LicT belongs to an essential family of bacterial antitermination proteins which bind to nascent mRNAs in order to stimulate transcription of sugar-metabolizing operons. As most of other antitermination protein...
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Article
Open AccessIntrinsic disorder in Viral Proteins Genome-Linked: experimental and predictive analyses
VPgs are viral proteins linked to the 5' end of some viral genomes. Interactions between several VPgs and eukaryotic translation initiation factors eIF4Es are critical for plant infection. However, VPgs are no...
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Article
Rapid Determination of Protein Solubility and Stability Conditions for NMR Studies Using Incomplete Factorial Design
Sample preparation constitutes a crucial and limiting step in structural studies of proteins by NMR. The determination of the solubility and stability (SAS) conditions of biomolecules at millimolar concentrati...
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Article
Letter to the Editor: 1H, 15N and 13C Backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein
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Article
Refined solution structure and backbone dynamics of 15N-labeled C12A-p8MTCP studied by NMR relaxation
MTCP1 (for Mature-T-Cell Proliferation) was the first gene unequivocally identified in the group of uncommon leukemias with a mature phenotype. The three-dimensional solution structure of the human p8MTCP protein...
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Article
Hyperthermostable Variants of a Highly Thermostable Alpha-Amylase
Genetic screening at temperatures between 70–80°C far exceeds the range of growth of most bacteria, and is not applicable to isolate easily thermostable protein variants. We describe a temperature shift protoc...