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Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC)
His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA...
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Metal-Mediated Ligand Affinity Chemistry (MLAC)
Metal-mediated ligand affinity chemistry (MLAC) enables site-specific protein modification and represents a powerful bioorthogonal strategy....
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Pilot investigation of magnetic nanoparticle–based immobilized metal affinity chromatography for efficient enrichment of phosphoproteoforms for mass spectrometry–based top-down proteomics
Protein phosphorylation is a vital and common post-translational modification (PTM) in cells, modulating various biological processes and diseases....
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Ti4+ functionalized β-cyclodextrin covalent organic framework as a new immobilized metal ion affinity chromatography platform for selective capture of phosphorylated peptides and exosomes
A Ti 4+ functionalized β-cyclodextrin covalent organic framework nanoparticle (named as β-CD-COF@Ti 4+ ) was synthesized using a one-pot method...
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Polyoxometalate functionalized magnetic metal–organic framework with multi-affinity sites for efficient enrichment of phosphopeptides
The reasonable design of metal–organic framework (MOF)–derived nanomaterial has important meaning in increasing the enrichment efficiency in the...
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Gas Chromatography
Gas chromatography (GC) has been used for the determination of a wide range of food components, but it is ideally suited for the analysis of...
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Ion Exchange Chromatography
Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to...
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Hydroxyapatite Chromatography (HAC)
Hydroxyapatite (HA) is a mixed-mode media that has been used extensively for the purification of proteins and DNA since the 1950s. Hydroxyapatite...
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Gas Chromatography
Gas chromatography is a type of chromatography in which the mobile phase.
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Antibody Purification Using Affinity Chromatography
Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently...
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Lectin Affinity Chromatography
Glycosylation is a non-template-driven posttranslational modification during which linked-sugars and glycans are added to the nascent polypeptide....
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Polymer brush grafted immobilized metal ion affinity adsorbent based on polydopamine/polyethyleneimine-coated magnetic graphene oxide for selective enrichment of cytokinins in plants
An immobilized metal affinity (IMAC) adsorbent was prepared for selective enrichment of adenine type CKs, via grafting polymer chain pendant with...
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Metal oxide–based macroporous ordered double affinity molecularly imprinted polymer for specific separation and enrichment of glycoprotein from food samples: a co-modification of DMSA and boronate affinity
Metal oxide–based macroporous ordered double affinity molecularly imprinted polymers (D-MIPs) were developed as solid phase extraction (SPE)...
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Specialised Forms of Chromatography
Even though adsorption chromatography is certainly by far the most widely used chromatographic separation method—not least because it is so versatile...
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Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation
Affinity chromatography is one of the versatile technique to selectively separate target biomolecules from complex biological sources (plasma,...
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Ion-Exchange Chromatography: Basic Principles and Application
Ion-exchange chromatography (IEC) is a fractionation technique that allows for the separation of ionizable molecules on the basis of differences in...
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Gas Chromatography and Mass Spectrometry: The Technique
Combining the techniques of gas chromatography and mass spectrometry provides conjunction with a confirmatory character, which has a great power of...
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Recent development and application of membrane chromatography
Membrane chromatography is mainly used for the separation and purification of proteins and biological macromolecules in the downstream processing...
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Liquid Chromatography in Bioanalysis
The extreme complexity of biological samples still poses a challenge in the resolution, identification, and quantification of their compounds. Liquid...
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Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors
Fibroblast growth factors (FGFs) are proteins with a vast array of biological activity, such as cell development and repair, glucose and bile acid...