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Antibody Purification Using Affinity Chromatography

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2466))

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Abstract

Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently contain other contaminants which may interfere with assays or cause adverse reactions if administered in vivo. Therefore, a means of isolating these antibodies from their source at high levels of purity is critical. Affinity chromatography is currently one of the most widely applied methods for the purification of antibodies. This method relies on specific and reversible, interactions between antibody structures, or recombinant tags fused to these structures, and ligands immobilized on solid support matrices, generally within a column. Herein, common chromatographic methods applied to antibody purification are described. These include the purification of IgG, and its recombinant forms, through protein A, protein G and immobilized metal affinity chromatography.

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Acknowledgments

This project was funded by the Department of Agriculture, Food and the Marine’s Competitive Research Funding Programme, Project ref. 15/S/618. This work was also supported by Science Foundation Ireland [Grant No. 14/IA/2646].

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Authors and Affiliations

  1. School of Biotechnology, Dublin City University, Dublin, Ireland

    Arabelle Cassedy & Richard O’Kennedy

  2. Hamad Bin Khalifa University, Doha, Qatar

    Richard O’Kennedy

  3. Qatar Foundation, Doha, Qatar

    Richard O’Kennedy

Authors
  1. Arabelle Cassedy
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  2. Richard O’Kennedy
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Corresponding author

Correspondence to Richard O’Kennedy .

Editor information

Editors and Affiliations

  1. Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA

    B. Vijayalakshmi Ayyar

  2. Astero Erado Inc, Houston, TX, USA

    Sushrut Arora

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Cassedy, A., O’Kennedy, R. (2022). Antibody Purification Using Affinity Chromatography. In: Ayyar, B.V., Arora, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 2466. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2176-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2176-9_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2175-2

  • Online ISBN: 978-1-0716-2176-9

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