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Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2466))

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Abstract

Affinity chromatography is one of the versatile technique to selectively separate target biomolecules from complex biological sources (plasma, saliva, urine, etc.). Conventional chromatography resins possess technical limitations at mini-analytical scale, which was overcome with the use of alternative material known as monoliths. This chapter discusses on the how to modify the fused silica capillary inner surface, prepare polymer monoliths within the capillary confinements, chelation of metal-ions on monoliths and protein separation from diluted human plasma using metal-ion monolith microcolumn.

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Acknowledgments

Dr. K. K. R. Tetala acknowledges SERB-DST, India for funding under two Schemes (SB/YS/LS-160/2014 and CRG/2018/003889) that facilitated the establishment of lab to pursue monolith development at miniaturized level.

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Authors and Affiliations

  1. Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India

    Kishore K. R. Tetala

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  1. Kishore K. R. Tetala
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Editors and Affiliations

  1. Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA

    B. Vijayalakshmi Ayyar

  2. Astero Erado Inc, Houston, TX, USA

    Sushrut Arora

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Tetala, K.K.R. (2022). Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation. In: Ayyar, B.V., Arora, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 2466. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2176-9_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2176-9_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2175-2

  • Online ISBN: 978-1-0716-2176-9

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