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Article
Glutamic Acid Signal Synchronizes Protein Synthesis Kinetics in Hepatocytes from Old Rats for the Following Several Days. Cell Metabolism Memory
The kinetics of protein synthesis was investigated in primary cultures of hepatocytes from old rats in serum–free medium. The rats were fed mixed fodder supplemented with glutamic acid and then transferred to ...
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Article
Glutamic acid as enhancer of protein synthesis kinetics in hepatocytes from old rats
Dense cultures of hepatocytes from old rats (~2 years old, body weight 530-610 g) are different from similar cultures of hepatocytes from young rats by the low amplitude of protein synthesis rhythm. Addition o...
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Article
Glutamic acid – amino acid, neurotransmitter, and drug – is responsible for protein synthesis rhythm in hepatocyte populations in vitro and in vivo
Primary cultures of rat hepatocytes were studied in serum-free media. Ultradian protein synthesis rhythm was used as a marker of cell synchronization in the population. Addition of glutamic acid (0.2 mg/ml) to...
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Article
Dopamine disorganizes direct intercellular interactions in keratinocytes cultures: A comparison to hepatocytes
Dopamine in the concentration 0.4 μg/mL abolishes protein synthesis rhythm in HaCaT keratinocytes and hepatocytes unlike noradrenaline or melatonin, which synchronize direct intercellular interactions and orga...
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Article
Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions
The effect of inhibition of proteasome activity on direct cell-cell interactions in primary hepatocyte cultures was studied. The circahoralian rhythm of protein synthesis was a marker of cell-cell communicatio...
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Article
Administration of Dopamine to Rats Disorganizes the Rhythm of Protein Synthesis in Hepatocytes
Dopamine was injected intravenously (9 μg/kg) or intraperitoneally (15 μg/kg) to Wistar rats (3-4 months, 300-400 g). Hepatocytes were isolated 40 min after dopamine injection. Dense cultures were maintained o...
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Article
Effect of Protein Synthesis Rhythm-Organizing Signal Persists for a Day after Single Administration of Melatonin to Rat
Melatonin administered to rat intraperitoneally organizes ultradian rhythm of protein synthesis in hepatocytes that persists for 1 day after exposure to the synchronizing signal. Hepatocytes were isolated 1 da...
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Article
Dopamine Disorganizes the Rhythm of Protein Synthesis Disrupting Self-Organization of Hepatocytes In Vitro
We studied dense 24-hour cultures of rat hepatocytes in serum-free medium on collagencoated slides. As before, a circahoralian rhythm of protein synthesis was observed in control cultures in a fresh medium. No...
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Article
The effect of the cell microenvironment on cell functions associated with tumor promotion and progression
To study the role of the cellular microenvironment in tumor promotion and progression, transformed cells of rat embryonic fibroblasts (CL-1 clone) were transplanted into transgenic immunodeficient mice and cel...
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Article
Effect of Transplantation of Bone Marrow Monomuclears on Angiogenesis in Rats
We studied the effect of non-selective intracoronary transplantation of bone marrow mononuclears on day 30 after acute coronary infarction on angiogenesis in rats. On days 14 and 30 after transplantation of mo...
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Article
Pathways of Bone Marrow Mononuclear Cell Differentiation after Transplantation into Postinfarction Heart
We studied homing and differentiation fate of transplanted bone marrow mononuclears after non-selective intracoronary injection on day 30 after acute myocardial infarction in rats. Mononuclear cells migrated t...
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Article
Activation of reconstructive processes in rat tissues under the action of radiofrequency current with a periodic impulse mode of modulation
The action of a current in the radio frequency range with a periodic impulse mode of modulation on the activation of recovery processes in the skin and skeletal muscles has been studied. The action of a radio ...
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Article
The State of Erythrocytes in Intact and Vagotomized Rats of Different Ages after Massive Hemorrhage
Acute massive hemorrhage in rats is accompanied by consistent changes in the morphofunctional characteristics of erythrocytes: diameter, area, polarization, form factor, and integral and specific optical densi...
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Article
Melatonin injected into rat efficiently synchronizes the rhythm of protein synthesis in primary hepatocyte cultures isolated from this rat
Melatonin injected intraperitoneally into rat synchronizes the ultradian rhythm of protein synthesis after 100 min in primary hepatocyte cultures isolated from this rat, which are studied after 1 or 2 days. Th...
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Article
Melatonin promotes and synchronizes protein synthesis in hepatocyte culture from old rats
The effect of 1 to 1000 nM melatonin was studied on daily cultures of rat hepatocytes on slides in serum-free medium. The minimum melatonin concentration (1 nM) proved to synchronize protein synthesis in async...
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Article
Development of differential sensitivity of CaOv ovarian adenocarcinoma cells to antitumor agents under conditions of hypoxia
We studied the role of VEGF signal pathway in autocrine regulation of tumor cell growth and survival under conditions of hypoxia. Hypoxia-resistant CaOv/H substrain with high level of VEGF-A secretion was obta...
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Article
The Role of the Vascular Factor in the Reorganization of Water Metabolism in Denervated Liver after Bacterial Endotoxin Poisoning
Intoxication with bacterial lipopolysaccharide (endotoxin) is accompanied by considerable rearrangements in the systems of blood microcirculation and water metabolism of the liver. These rearrangements are man...
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Article
Differences in Lipid Composition and Proliferative Activity of Rat Hepatoma-27 Depending on the Target Organ
Proliferative activity and lipid composition (phospholipids and gangliosides) were studied in rat hepatoma-27 transplanted subcutaneously or intrahepatically (as models for primary and metastasizing tumors). T...
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Article
Activity of detoxication enzymes in rat hepatoma 27 transplanted in different organs
Activities of enzymes of active oxygen forms detoxication and phase II xenobiotic metabolism were measured in rat hepatoma 27 cells transplanted to different organs. Activity of phase II xenobiotic metabolizin...
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Article
Activity of detoxication enzymes in rat hepatoma 27 transplanted in different organs
Activities of enzymes of active oxygen forms detoxication and phase II xenobiotic metabolism were measured in rat hepatoma 27 cells transplanted to different organs. Activity of phase II xenobiotic metabolizin...
