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Article
Glutamic Acid Signal Synchronizes Protein Synthesis Kinetics in Hepatocytes from Old Rats for the Following Several Days. Cell Metabolism Memory
The kinetics of protein synthesis was investigated in primary cultures of hepatocytes from old rats in serum–free medium. The rats were fed mixed fodder supplemented with glutamic acid and then transferred to ...
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Article
Glutamic acid as enhancer of protein synthesis kinetics in hepatocytes from old rats
Dense cultures of hepatocytes from old rats (~2 years old, body weight 530-610 g) are different from similar cultures of hepatocytes from young rats by the low amplitude of protein synthesis rhythm. Addition o...
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Article
Glutamic acid – amino acid, neurotransmitter, and drug – is responsible for protein synthesis rhythm in hepatocyte populations in vitro and in vivo
Primary cultures of rat hepatocytes were studied in serum-free media. Ultradian protein synthesis rhythm was used as a marker of cell synchronization in the population. Addition of glutamic acid (0.2 mg/ml) to...
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Article
Dopamine disorganizes direct intercellular interactions in keratinocytes cultures: A comparison to hepatocytes
Dopamine in the concentration 0.4 μg/mL abolishes protein synthesis rhythm in HaCaT keratinocytes and hepatocytes unlike noradrenaline or melatonin, which synchronize direct intercellular interactions and orga...
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Article
Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions
The effect of inhibition of proteasome activity on direct cell-cell interactions in primary hepatocyte cultures was studied. The circahoralian rhythm of protein synthesis was a marker of cell-cell communicatio...
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Article
Administration of Dopamine to Rats Disorganizes the Rhythm of Protein Synthesis in Hepatocytes
Dopamine was injected intravenously (9 μg/kg) or intraperitoneally (15 μg/kg) to Wistar rats (3-4 months, 300-400 g). Hepatocytes were isolated 40 min after dopamine injection. Dense cultures were maintained o...
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Article
Effect of Protein Synthesis Rhythm-Organizing Signal Persists for a Day after Single Administration of Melatonin to Rat
Melatonin administered to rat intraperitoneally organizes ultradian rhythm of protein synthesis in hepatocytes that persists for 1 day after exposure to the synchronizing signal. Hepatocytes were isolated 1 da...
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Article
Dopamine Disorganizes the Rhythm of Protein Synthesis Disrupting Self-Organization of Hepatocytes In Vitro
We studied dense 24-hour cultures of rat hepatocytes in serum-free medium on collagencoated slides. As before, a circahoralian rhythm of protein synthesis was observed in control cultures in a fresh medium. No...
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Article
Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal
A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were...
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Article
Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes
In cultures of human keratinocytes HaCaT contained in a serum-free medium on glass, a circahoralian rhythm of protein synthesis was found similar to the one in hepatocytes in vitro. The intensity of the synthe...
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Article
Melatonin injected into rat efficiently synchronizes the rhythm of protein synthesis in primary hepatocyte cultures isolated from this rat
Melatonin injected intraperitoneally into rat synchronizes the ultradian rhythm of protein synthesis after 100 min in primary hepatocyte cultures isolated from this rat, which are studied after 1 or 2 days. Th...
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Article
Melatonin synchronizes protein synthesis rhythm in hepatocyte cultures as an agonist of intercellular calcium and protein kinases
Melatonin in nanomolar concentrations synchronizes protein synthesis in primary cultures of hepatocytes through calcium-dependent activation of protein kinases. The synchronizing effect of melatonin is blocked...
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Article
Melatonin promotes and synchronizes protein synthesis in hepatocyte culture from old rats
The effect of 1 to 1000 nM melatonin was studied on daily cultures of rat hepatocytes on slides in serum-free medium. The minimum melatonin concentration (1 nM) proved to synchronize protein synthesis in async...
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Article
Signaling factors of self-organization of protein synthesis rhythm in hepatocyte cultures—gangliosides and catecholamines function independently
Considering the data on the low level of self-organization (self-synchronization) of protein synthesis rhythm in aging, we studied the possible interference of the signaling factors of self-organization, gangl...
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Article
Mechanism of direct cell interactions. Self-organization of protein synthesis rhythm
Primary 24-hour cultures of hepatocytes on slides in a serum-free medium were studied. Circahoralian rhythm of protein synthesis served as a marker of cell cooperation. Stimulation of protein kinase activities...
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Article
Aftereffect of synchronizers of protein synthesis rhythm in hepatocytes in vitro
The effect of gangliosides and phenylephrine synchronizing the protein synthesis rhythm was preserved in hepatocytes cultured in the normal serum-free medium for one-two days. Hence, the membrane signal trigge...
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Article
Age-related features of protein synthesis rhythm. Effects of extracellular medium
Cell interactions have been studied in cultures pf hepatocytes from young and old rats. The rhythm of protein synthesis is an index of cell interaction and synchronization in culture, while the amplitude of os...
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Article
Cooperation of Hepatocytes in vitro in the Rhythm of Protein Synthesis is Promoted by the GM1 Ganglioside Contained in Vesicles and Liposomes
The medium conditioned by dense, self-synchronized hepatocyte cultures was centrifuged at 150 000 g to obtain two fractions. The light fraction (supernatant fluid) contained ganglioside monomers and micelles, ...
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Article
Disturbed Cooperation of Hepatocytes in the Rhythm of Protein Synthesis by Chelating Agent of Cytoplasmic Calcium BAPTA-AM
Previously we demonstrated synchronized oscillations of protein synthesis rate in hepatocyte cultures upon accumulation of monosialoganglioside GM1 in the medium or after introduction of exogenous GM1 to the m...
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Article
Effect of gangliosides secreted by ascites hepatoma 22a cells on intensity of protein synthesis in these cells and on their sensitivity to indocarb
