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Article
Open AccessComparison of bleb morphologies between phacoemulsification combined with Ex-PRESS mini shunt implantation, phacotrabeculectomy and trabeculectomy alone: a two-year retrospective in vivo confocal microscopy study
To compare the bleb morphologies of phacoemulsification combined with Ex-PRESS implantation (Phaco-ExPRESS), phaco trabeculectomy (Phaco-Trab), and trabeculectomy (Trab) in postoperative two years.
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Article
Open AccessA framework for Frizzled-G protein coupling and implications to the PCP signaling pathways
The ten Frizzled receptors (FZDs) are essential in Wnt signaling and play important roles in embryonic development and tumorigenesis. Among these, FZD6 is closely associated with lens development. Understandin...
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Article
Open AccessChemical-induced phase transition and global conformational reorganization of chromatin
Chemicals or drugs can accumulate within biomolecular condensates formed through phase separation in cells. Here, we use super-resolution imaging to search for chemicals that induce phase transition within chr...
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Article
Alternative Z-genome biosynthesis pathway shows evolutionary progression from Archaea to phage
Many bacteriophages evade bacterial immune recognition by substituting adenine with 2,6-diaminopurine (Z) in their genomes. The Z-genome biosynthetic pathway involves PurZ that belongs to the PurA (adenylosucc...
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Article
Open AccessStructural insights into the human niacin receptor HCA2-Gi signalling complex
The hydroxycarboxylic acid receptor 2 (HCA2) agonist niacin has been used as treatment for dyslipidemia for several decades albeit with skin flushing as a common side-effect in treated individuals. Extensive e...
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Article
Open AccessRational design of GDP‑d‑mannose mannosyl hydrolase for microbial l‑fucose production
l‑Fucose is a rare sugar that has beneficial biological activities, and its industrial production is mainly achieved with brown algae through acidic/enzymatic fucoidan hydrolysis and a cumbersome purification p.....
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Article
Open AccessThe activation mechanism and antibody binding mode for orphan GPR20
GPR20 is a class-A orphan G protein-coupled receptor (GPCR) and a potential therapeutic target for gastrointestinal stromal tumors (GIST) owing to its differentially high expression. An antibody-drug conjugate...
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Article
Open AccessCryo-EM structures of orphan GPR21 signaling complexes
GPR21 is a class-A orphan G protein-coupled receptor (GPCR) and a potential therapeutic target for type 2 diabetes and other metabolic disorders. This receptor shows high basal activity in coupling to multiple...
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Article
Building the Chordata Olfactory Receptor Database using more than 400,000 receptors annotated by Genome2OR
Olfactory receptors are poorly annotated for most genome-sequenced chordates. To address this deficiency, we developed a nhmmer-based olfactory receptor annotation tool Genome2OR (https://github....
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Article
Cytoophidia coupling adipose architecture and metabolism
Tissue architecture determines its unique physiology and function. How these properties are intertwined has remained unclear. Here we show that the metabolic enzyme CTP synthase (CTPS) form filamentous structu...
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Article
H2B Lys34 Ubiquitination Induces Nucleosome Distortion to Stimulate Dot1L Activity
Ubiquitination-dependent histone crosstalk plays critical roles in chromatin-associated processes and is highly associated with human diseases. Mechanism studies of the crosstalk have been of the central focus...
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Article
Target tracking algorithm based on multiscale analysis and combinatorial matching
Aiming at improving the accuracy of current tracking algorithms and tracking the target more quickly, a new target tracking method that is from multiscale analysis is presented, which is combined with combinat...
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Article
Open AccessThe unconventional activation of the muscarinic acetylcholine receptor M4R by diverse ligands
Muscarinic acetylcholine receptors (mAChRs) respond to the neurotransmitter acetylcholine and play important roles in human nervous system. Muscarinic receptor 4 (M4R) is a promising drug target for treating n...
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Article
Structures of the human cholecystokinin receptors bound to agonists and antagonists
Cholecystokinin receptors, CCKAR and CCKBR, are important neurointestinal peptide hormone receptors and play a vital role in food intake and appetite regulation. Here, we report three crystal structures of the hu...
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Article
A semi-supervised self-training method based on density peaks and natural neighbors
The semi-supervised self-training method is one of the successful methodologies of semi-supervised classification and can train a classifier by exploiting both labeled data and unlabeled data. However, most of...
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Article
Open AccessG protein-coupled receptors: structure- and function-based drug discovery
As one of the most successful therapeutic target families, G protein-coupled receptors (GPCRs) have experienced a transformation from random ligand screening to knowledge-driven drug design. We are eye-witness...
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Article
Open AccessA unique hormonal recognition feature of the human glucagon-like peptide-2 receptor
Glucagon-like peptides (GLP-1 and GLP-2) are two proglucagon-derived intestinal hormones that mediate distinct physiological functions through two related receptors (GLP-1R and GLP-2R) which are important drug...
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Article
Open AccessStructural basis for activation of the growth hormone-releasing hormone receptor
Growth hormone-releasing hormone (GHRH) regulates the secretion of growth hormone that virtually controls metabolism and growth of every tissue through its binding to the cognate receptor (GHRHR). Malfunction ...
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Article
Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
Cas9 nucleases recognize and cleave their target DNA through base pairing of a guide RNA with a spacer adjacent to a protospacer adjacent motif (PAM). Streptococcus thermophilus Cas9 (St1Cas9), a smaller Cas9 ort...
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Article
Structural basis of CXC chemokine receptor 2 activation and signalling
Chemokines and their receptors mediate cell migration, which influences multiple fundamental biological processes and disease conditions such as inflammation and cancer1. Although ample effort has been invested i...
