13 Result(s)

Article

A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function

Antigen stimulation of immune cells triggers Ca²⁺ entry through Ca²⁺ release-activated Ca²⁺ (CRAC) channels, promoting the immune response to pathogens by activating the transcription factor NFAT. We have previou...

Stefan Feske, Yousang Gwack, Murali Prakriya, Sonal Srikanth, Sven-Holger Puppel… in Nature (2006)

Article

A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT

Down's syndrome is caused by an extra chromosome; somehow a 1.5-fold increase in the dosage of a gene or genes on chromosome 21 causes the wide-reaching effects associated with the condition. A study using ‘kn...

Yousang Gwack, Sonia Sharma, Julie Nardone, Bogdan Tanasa, Alina Iuga… in Nature (2006)

Article

Orai1 is an essential pore subunit of the CRAC channel

Two groups report the molecular identification of the long-sought CRAC channel as a plasma membrane protein known variously as olf186-F, Orai and CRACM1. The CRAC channel is of fundamental importance to Ca²⁺ sign...

Murali Prakriya, Stefan Feske, Yousang Gwack, Sonal Srikanth, Anjana Rao… in Nature (2006)

Article

A novel EF-hand protein, CRACR2A, is a cytosolic Ca²⁺ sensor that stabilizes CRAC channels in T cells

The calcium-binding protein CRACR2A positively regulates the activity of CRAC channels. CRACR2A is a ternary complex which binds to, and stabilizes, the interaction between STIM1 and Orai1, and disassociates a...

Sonal Srikanth, Hea-** Jung, Kyun-Do Kim, Puneet Souda… in Nature Cell Biology (2010)

Protocol

Measurement of Intracellular Ca²⁺ Concentration in Single Cells Using Ratiometric Calcium Dyes

Measurement of intracellular Ca²⁺ concentration ([Ca²⁺]_i) is useful to study the upstream and downstream events of Ca²⁺ signaling. Ca²⁺-binding proteins including EF-hand-containing proteins are important downstr...

Sonal Srikanth, Yousang Gwack in Calcium-Binding Proteins and RAGE (2013)

Article

Orai1-NFAT signalling pathway triggered by T cell receptor stimulation

T cell receptor (TCR) stimulation plays a crucial role in development, homeostasis, proliferation, cell death, cytokine production, and differentiation of T cells. Thus, in depth understanding of TCR signallin...

Sonal Srikanth, Yousang Gwack in Molecules and Cells (2013)

Article

The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4⁺ T cells

The membrane channel TRPV1 is well studied as a pain receptor, but its function beyond sensory neurons remains unclear. Raz and colleagues demonstrate that TRPV1 is also a non–store-operated calcium channel on...

Samuel Bertin, Yukari Aoki-Nonaka, Petrus Rudolf de Jong… in Nature Immunology (2014)

Chapter

Immunological Disorders: Regulation of Ca²⁺ Signaling in T Lymphocytes

Engagement of T cell receptors (TCRs) with cognate antigens triggers cascades of signaling pathways in helper T cells. TCR signaling is essential for the effector function of helper T cells including prolifera...

Sonal Srikanth, ** Seok Woo, Zuoming Sun… in Store-Operated Ca²⁺ Entry (SOCE) Pathways (2017)

Article

The Ca²⁺ sensor STIM1 regulates the type I interferon response by retaining the signaling adaptor STING at the endoplasmic reticulum

Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER) signaling adaptor that is essential for the type I interferon response to DNA pathogens. Aberrant activation of STING is linked to the pa...

Sonal Srikanth, ** Seok Woo, Beibei Wu, Yasser M. El-Sherbiny… in Nature Immunology (2019)

Article

A Report of Novel STIM1 Deficiency and 6-Year Follow-Up of Two Previous Cases Associated with Mild Immunological Phenotype

Laura Rice, Claire Stockdale, Ian Berry, Sean O’Riordan… in Journal of Clinical Immunology (2019)