-
Article
Open AccessChromatin accessibility during human first-trimester neurodevelopment
The human brain develops through a tightly organized cascade of patterning events, induced by transcription factor expression and changes in chromatin accessibility. Although gene expression across the develop...
-
Article
Spatial tissue profiling by imaging-free molecular tomography
Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe...
-
Article
Molecular architecture of the develo** mouse brain
The mammalian brain develops through a complex interplay of spatial cues generated by diffusible morphogens, cell–cell interactions and intrinsic genetic programs that result in probably more than a thousand d...
-
Article
Publisher Correction: Altered perivascular fibroblast activity precedes ALS disease onset
-
Article
Altered perivascular fibroblast activity precedes ALS disease onset
Apart from well-defined factors in neuronal cells1, only a few reports consider that the variability of sporadic amyotrophic lateral sclerosis (ALS) progression can depend on less-defined contributions from glia2
-
Article
Open AccessSingle cell transcriptomics of primate sensory neurons identifies cell types associated with chronic pain
Distinct types of dorsal root ganglion sensory neurons may have unique contributions to chronic pain. Identification of primate sensory neuron types is critical for understanding the cellular origin and herita...
-
Article
RNA velocity of single cells
RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and throughput1. However, this approach cap...
-
Article
Neuronal atlas of the dorsal horn defines its architecture and links sensory input to transcriptional cell types
The dorsal horn of the spinal cord is critical to processing distinct modalities of noxious and innocuous sensation, but little is known of the neuronal subtypes involved, hampering efforts to deduce principle...
-
Article
Conserved properties of dentate gyrus neurogenesis across postnatal development revealed by single-cell RNA sequencing
The dentate gyrus of the hippocampus is a brain region in which neurogenesis persists into adulthood; however, the relationship between developmental and adult dentate gyrus neurogenesis has not been examined ...
-
Article
Open AccessSTRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT...
-
Article
Presymptomatic activation of the PDGF-CC pathway accelerates onset of ALS neurodegeneration
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with unknown origins. Neurodegeneration in ALS mouse models occurs together with signs of disrupted blood–spinal cord barrier (BSCB) and...
-
Article
Open AccessGene expression analysis of skin grafts and cultured keratinocytes using synthetic RNA normalization reveals insights into differentiation and growth control
Keratinocytes (KCs) are the most frequent cells in the epidermis, and they are often isolated and cultured in vitro to study the molecular biology of the skin. Cultured primary cells and various immortalized cell...
-
Article
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing
Based on single cell RNA-sequencing of 622 adult mouse sensory neurons, Usoskin et al. performed unbiased classification to identify the cellular and molecular complexity underlying somatic sensation. Eleven diff...
-
Article
Open AccessTopographical transcriptome map** of the mouse medial ganglionic eminence by spatially resolved RNA-seq
Cortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventri...
-
Article
Quantitative single-cell RNA-seq with unique molecular identifiers
With an optimized protocol and unique molecular identifiers (UMIs) to tag individual transcripts, the mRNA complement of a single cell can be quantified on an absolute scale with almost no amplification bias.
-
Article
Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing
Single-cell analysis of gene expression is increasingly important for the analysis of complex tissues, including cancer, develo** organs and adult stem cell niches. Here we present a detailed protocol for qu...
-
Article
Rapid genome sequencing with short universal tiling probes
The increasing availability of high-quality reference genomic sequences has created a demand for ways to survey the sequence differences present in individual genomes. Here we describe a DNA sequencing method ...
-
Article
Engineering cells to secrete growth factors
The neutrotrophins stimulate survival and differentiation of a range of target neurons. A wealth of evidence suggests that central cholinergic neurons depend on nerve growth factor (NGF) for trophic support. G...
