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  1. Article

    Differential regulation of mouse pancreatic islet insulin secretion and Smad proteins by activin ligands

    Glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells is regulated by paracrine factors, the identity and mechanisms of action of which are incompletely understood. Activins are expressed in p...

    Haiya Wu, Karima Mezghenna, Patricia Marmol, Tingqing Guo, Annalena Moliner… in Diabetologia (2014)

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    Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing

    Single-cell analysis of gene expression is increasingly important for the analysis of complex tissues, including cancer, develo** organs and adult stem cell niches. Here we present a detailed protocol for qu...

    Saiful Islam, Una Kjällquist, Annalena Moliner, Pawel Zajac… in Nature Protocols (2012)

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    Optimized mouse ES cell culture system by suspension growth in a fully defined medium

    Mouse and human embryonic stem (mES and hES) cells have become one of the most intensively studied primary cell types in biomedical research. However, culturing ES cells is notoriously labor intensive. We have...

    Michael Andäng, Annalena Moliner, Claudia A Doege, Carlos F Ibañez… in Nature Protocols (2008)

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    Histone H2AX-dependent GABAA receptor regulation of stem cell proliferation

    Stem cell self-renewal involves the maintenance of pluripotency under continued proliferation. Proliferation of embryonic stem cells was thought to be constitutive and not to be regulated, because the normal c...

    Michael Andäng, Jens Hjerling-Leffler, Annalena Moliner, T. Kalle Lundgren… in Nature (2008)