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Open AccessBrain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS function
The myelinated white matter tracts of the central nervous system (CNS) are essential for fast transmission of electrical impulses and are often differentially affected in human neurodegenerative diseases acros...
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Article
The lipotype hypothesis
Multicellular organisms consist of different cell types that emerge in response to instructive signals. New evidence suggests that lipid composition modulates cell response to signalling cues. Here we propose ...
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Open AccessClonal relations in the mouse brain revealed by single-cell and spatial transcriptomics
The mammalian brain contains many specialized cells that develop from a thin sheet of neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of molecularly diverse cell types in the ne...
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Open AccessConfronting false discoveries in single-cell differential expression
Differential expression analysis in single-cell transcriptomics enables the dissection of cell-type-specific responses to perturbations such as disease, trauma, or experimental manipulations. While many statis...
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Spatial tissue profiling by imaging-free molecular tomography
Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe...
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Molecular architecture of the develo** mouse brain
The mammalian brain develops through a complex interplay of spatial cues generated by diffusible morphogens, cell–cell interactions and intrinsic genetic programs that result in probably more than a thousand d...
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Cell type prioritization in single-cell data
We present Augur, a method to prioritize the cell types most responsive to biological perturbations in single-cell data. Augur employs a machine-learning framework to quantify the separability of perturbed and...
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Open AccessMyocardial micro-biopsy procedure for molecular characterization with increased precision and reduced trauma
Endomyocardial biopsy is a valuable tool in cardiac diagnostics but is limited by low diagnostic yield and significant complication risks. Meanwhile, recent developments in transcriptomic and proteomic technol...
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From single-cell RNA-seq to transcriptional regulation
Analysis of gene expression and chromatin accessibility in single cells provides insight into development and disease.
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Open AccessA cell fitness selection model for neuronal survival during development
Developmental cell death plays an important role in the construction of functional neural circuits. In vertebrates, the canonical view proposes a selection of the surviving neurons through stochastic competiti...
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Spatial organization of the somatosensory cortex revealed by osmFISH
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new metho...
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RNA velocity of single cells
RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and throughput1. However, this approach cap...
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Neuronal atlas of the dorsal horn defines its architecture and links sensory input to transcriptional cell types
The dorsal horn of the spinal cord is critical to processing distinct modalities of noxious and innocuous sensation, but little is known of the neuronal subtypes involved, hampering efforts to deduce principle...
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Open AccessSTRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT...
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Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model
In vivo reprogramming of astrocytes to dopamine neurons improves motor behavior in a mouse model of Parkinson's disease.
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Visceral motor neuron diversity delineates a cellular basis for nipple- and pilo-erection muscle control
The sympathetic system maintains a physiological balance, adjusts bodily functions during daily living activities, and can activate stress responses. The authors identify a variety of unique sympathetic neuron...
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Quantitative single-cell RNA-seq with unique molecular identifiers
With an optimized protocol and unique molecular identifiers (UMIs) to tag individual transcripts, the mRNA complement of a single cell can be quantified on an absolute scale with almost no amplification bias.
