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Article
Open AccessOctyl itaconate enhances VSVΔ51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways
The presence of heterogeneity in responses to oncolytic virotherapy poses a barrier to clinical effectiveness, as resistance to this treatment can occur through the inhibition of viral spread within the tumor,...
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Article
Open AccessThe proteasome modulates endocytosis specifically in glomerular cells to promote kidney filtration
Kidney filtration is ensured by the interaction of podocytes, endothelial and mesangial cells. Immunoglobulin accumulation at the filtration barrier is pathognomonic for glomerular injury. The mechanisms that ...
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Article
Open AccessIn vivo characterization of a podocyte-expressed short podocin isoform
The most common genetic causes of steroid-resistant nephrotic syndrome (SRNS) are mutations in the NPHS2 gene, which encodes the cholesterol-binding, lipid-raft associated protein podocin. Mass spectrometry and c...
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Article
Open AccessLong-read sequencing identifies a common transposition haplotype predisposing for CLCNKB deletions
Long-read sequencing is increasingly used to uncover structural variants in the human genome, both functionally neutral and deleterious. Structural variants occur more frequently in regions with a high homolog...
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Article
Open AccessAn integrated organoid omics map extends modeling potential of kidney disease
Kidney organoids are a promising model to study kidney disease, but their use is constrained by limited knowledge of their functional protein expression profile. Here, we define the organoid proteome and trans...
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Article
Open AccessAccelerated lysine metabolism conveys kidney protection in salt-sensitive hypertension
Hypertension and kidney disease have been repeatedly associated with genomic variants and alterations of lysine metabolism. Here, we combined stable isotope labeling with untargeted metabolomics to investigate...
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Article
Open AccessProteolysis and inflammation of the kidney glomerulus
Proteases play a central role in regulating renal pathophysiology and are increasingly evaluated as actionable drug targets. Here, we review the role of proteolytic systems in inflammatory kidney disease. Infl...
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Article
The tissue proteome in the multi-omic landscape of kidney disease
Kidney research is entering an era of ‘big data’ and molecular omics data can provide comprehensive insights into the molecular footprints of cells. In contrast to transcriptomics, proteomics and metabolomics ...
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Article
Cognitive analysis of metabolomics data for systems biology
Cognitive computing is revolutionizing the way big data are processed and integrated, with artificial intelligence (AI) natural language processing (NLP) platforms hel** researchers to efficiently search and...
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Article
Open AccessThe ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells
Histones modulate gene expression by chromatin compaction, regulating numerous processes such as differentiation. However, the mechanisms underlying histone degradation remain elusive. Human embryonic stem cel...
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Article
A molecular mechanism explaining albuminuria in kidney disease
Mammalian kidneys constantly filter large amounts of liquid, with almost complete retention of albumin and other macromolecules in the plasma. Breakdown of the three-layered renal filtration barrier results in...
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Article
An integrative approach to cisplatin chronic toxicities in mice reveals importance of organic cation-transporter-dependent protein networks for renoprotection
Cisplatin (CDDP) is one of the most important chemotherapeutic drugs in modern oncology. However, its use is limited by severe toxicities, which impair life quality after cancer. Here, we investigated the role...
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Article
Identification of bioactive metabolites using activity metabolomics
The metabolome, the collection of small-molecule chemical entities involved in metabolism, has traditionally been studied with the aim of identifying biomarkers in the diagnosis and prediction of disease. Howe...
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Article
Open AccessUrine-derived cells: a promising diagnostic tool in Fabry disease patients
Fabry disease is a lysosomal storage disorder resulting from impaired alpha-galactosidase A (α-Gal A) enzyme activity due to mutations in the GLA gene. Currently, powerful diagnostic tools and in vivo research mo...
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Article
Open AccessTargeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus
Ciliopathies comprise a large number of hereditary human diseases and syndromes caused by mutations resulting in dysfunction of either primary or motile cilia. Both types of cilia share a similar architecture....
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Chapter
Overview
The kidney serves important functions for body homeostasis, including (1) regulation of water and electrolyte metabolism, (2) regulation of acid-base homeostasis, (3) detoxification and excretion of waste prod...
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Article
Open AccessCharacterization of a short isoform of the kidney protein podocin in human kidney
Steroid resistant nephrotic syndrome is a severe hereditary disease often caused by mutations in the NPHS2 gene. This gene encodes the lipid binding protein podocin which localizes to the slit diaphragm of podocy...
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Article
Different effects of CsA and FK506 on aquaporin-2 abundance in rat primary cultured collecting duct cells
Calcineurin (Cn) inhibitors (CnI) such as cyclosporine A (CsA) and FK506 are nephrotoxic immunosuppressant drugs, which decrease tubular function. Here, we examined the direct effect of CnI on aquaporin-2 (AQP...
