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6 Result(s)

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  1. No Access

    Article

    A Simple and Effective Method for Speciation Analysis of 13 Arsenic Species Using HPLC on a Fluorocarbon Stationary Phase Coupled to ICP-MS

    The separation properties of arsenic species was investigated using HPLC-ICP-MS with several commercially available fluorocarbon stationary phases and no ion-pair reagents in HPLC. One pentafluorophenyl column...

    Shin-ichi Miyashita, Kenji Kinoshita, Toshikazu Kaise in Analytical Sciences (2021)

  2. No Access

    Article

    Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction

    We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new...

    Mariko Hayashida, Tomoko Ota, Minori Ishii, Kyoko Iwao-Koizumi… in Analytical Sciences (2014)

  3. No Access

    Article

    Genoty** of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction

    We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genoty** method that works directly on whole human blood. This single-tube genoty** method was used to ...

    Mariko Hayashida, Kyoko Iwao-Koizumi, Shigenori Murata… in Analytical Sciences (2010)

  4. No Access

    Article

    Single-Tube Genoty** from a Human Hair Root by Direct PCR

    We have developed a simple, labor-saving, inexpensive and rapid SNP genoty** method that directly uses a human hair root as the template. This single-tube genoty** method was used to successfully and relia...

    Mariko Hayashida, Kyoko Iwao-Koizumi, Shigenori Murata… in Analytical Sciences (2009)

  5. No Access

    Article

    All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer

    In this report, we have developed a novel quantitative RT-PCR protocol in which the procedure including mRNA purification can be performed in an all-in-one tube. To simplify gene expression analysis, oligo-dT30 i...

    Atsuko Tanaka, Naoki Harikai, Shin Saito, Toru Yakabe, Sohei Funaoka… in Analytical Sciences (2009)

  6. No Access

    Article

    Reliable and Fast Allele-Specific Extension of 3′-LNA Modified Oligonucleotides Covalently Immobilized on a Plastic Base, Combined with Biotin-dUTP Mediated Optical Detection

    In the present work, a convenient microarray SNP ty** system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achiev...

    Yuichi Michikawa, Kentaro Fujimoto, Kenji Kinoshita, Seiko Kawai… in Analytical Sciences (2006)