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Article
A Simple and Effective Method for Speciation Analysis of 13 Arsenic Species Using HPLC on a Fluorocarbon Stationary Phase Coupled to ICP-MS
The separation properties of arsenic species was investigated using HPLC-ICP-MS with several commercially available fluorocarbon stationary phases and no ion-pair reagents in HPLC. One pentafluorophenyl column...
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Article
Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new...
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Article
Genoty** of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genoty** method that works directly on whole human blood. This single-tube genoty** method was used to ...
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Article
Single-Tube Genoty** from a Human Hair Root by Direct PCR
We have developed a simple, labor-saving, inexpensive and rapid SNP genoty** method that directly uses a human hair root as the template. This single-tube genoty** method was used to successfully and relia...
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Article
All-in-One Tube Method for Quantitative Gene Expression Analysis in Oligo-dT30 Immobilized PCR Tube Coated with MPC Polymer
In this report, we have developed a novel quantitative RT-PCR protocol in which the procedure including mRNA purification can be performed in an all-in-one tube. To simplify gene expression analysis, oligo-dT30 i...
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Article
Adsorption of 4-nonylphenol ethoxylates onto insoluble chitosan beads bearing cyclodextrin moieties
In order to develop a treatment method for industrial wastewater, the adsorption of 4-nonylphenol ethoxylates (NPEs), non-ionic surfactants used in the industry, onto chitosan beads having cyclodextrin (CDC be...
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Article
Reliable and Fast Allele-Specific Extension of 3′-LNA Modified Oligonucleotides Covalently Immobilized on a Plastic Base, Combined with Biotin-dUTP Mediated Optical Detection
In the present work, a convenient microarray SNP ty** system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achiev...