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Article
Open AccessStructural role for DNA Ligase IV in promoting the fidelity of non-homologous end joining
Nonhomologous end joining (NHEJ), the primary pathway of vertebrate DNA double-strand-break (DSB) repair, directly re-ligates broken DNA ends. Damaged DSB ends that cannot be immediately re-ligated are modifie...
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Article
Open AccessCooperative assembly of p97 complexes involved in replication termination
The p97 ATPase extracts polyubiquitylated proteins from diverse cellular structures in preparation for destruction by the proteasome. p97 functions with Ufd1-Npl4 and a variety of UBA-UBX co-factors, but how p...
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Article
CDC7-independent G1/S transition revealed by targeted protein degradation
The entry of mammalian cells into the DNA synthesis phase (S phase) represents a key event in cell division1. According to current models of the cell cycle, the kinase CDC7 constitutes an essential and rate-limit...
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Article
The HMCES DNA-protein cross-link functions as an intermediate in DNA interstrand cross-link repair
The 5-hydroxymethylcytosine binding, embryonic stem-cell-specific (HMCES) protein forms a covalent DNA-protein cross-link (DPC) with abasic (AP) sites in single-stranded DNA, and the resulting HMCES-DPC is tho...
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Article
ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation
Cells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII u...
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Article
Open AccessPublisher Correction: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
A new varietal of DNA interstrand crosslink repair
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Article
Open AccessThe cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA...
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Article
TRAIP is a master regulator of DNA interstrand crosslink repair
Cells often use multiple pathways to repair the same DNA lesion, and the choice of pathway has substantial implications for the fidelity of genome maintenance. DNA interstrand crosslinks covalently link the tw...
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Article
A single XLF dimer bridges DNA ends during nonhomologous end joining
Nonhomologous end joining (NHEJ) is the primary pathway of DNA double-strand-break repair in vertebrate cells, yet how NHEJ factors assemble a synaptic complex that bridges DNA ends remains unclear. To address...
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Article
Mechanisms of DNA replication termination
Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessa...
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Protocol
Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
Faithful chromosome segregation during cell division requires proper bipolar spindle assembly and critically depends on spindle pole integrity. In most animal cells, spindle poles form as the result of the con...
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Article
The mechanism of DNA replication termination in vertebrates
Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge. Termination involves local completion of DNA synthesis, decatenation of daughter molecules and replisome disass...
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Article
DNA interstrand cross-link repair requires replication-fork convergence
New biochemical analyses in Xenopus cell-free extracts show that two replication forks must converge on a DNA interstrand cross-link (ICL) to permit translesion synthesis and repair.
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Article
Prereplication-complex formation: a molecular double take?
DNA replication begins with prereplication-complex formation at origins and is followed by helicase activation to unwind DNA at the replication fork. This Perspective compares bacterial DnaB and eukaryotic MCM...
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Article
Bypass of a protein barrier by a replicative DNA helicase
Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (known as steric exclusion). By contra...
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Article
A general approach to break the concentration barrier in single-molecule imaging
A method for single-molecule imaging at high protein concentrations is presented and demonstrated in the context of DNA replication in cell extracts.
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Protocol
Replication-Coupled DNA Interstrand Cross-Link Repair in Xenopus Egg Extracts
Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA ...
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Protocol
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for prepara...
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Protocol
DNA Replication in Nucleus-Free Xenopus Egg Extracts
Extracts derived from Xenopus laevis eggs represent a powerful cell-free system to study eukaryotic DNA replication. A variation of the system allows for DNA replication not only in a cell-free environment, but a...
