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Article
Open AccessExperimental and numerical investigation of polymer pore-clogging in micromodels
Polymers have been used effectively in the Oil & Gas Industry for a variety of field applications, such as enhanced oil recovery (EOR), well conformance, mobility control, and others. Polymer intermolecular in...
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Article
Open AccessBioengineering of air-filled protein nanoparticles by genetic and chemical functionalization
Various bacteria and archaea, including halophilic archaeon Halobacterium sp. NRC-1 produce gas vesicle nanoparticles (GVNPs), a unique class of stable, air-filled intracellular proteinaceous nanostructures. GVNP...
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Article
Open AccessSingle-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling
Hematopoietic stem/progenitor cell (HSPC) and leukemic cell homing is an important biological phenomenon that occurs through key interactions between adhesion molecules. Tethering and rolling of the cells on e...
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Article
Open AccessInitial state of DNA-Dye complex sets the stage for protein induced fluorescence modulation
Protein-induced fluorescence enhancement (PIFE) is a popular tool for characterizing protein-DNA interactions. PIFE has been explained by an increase in local viscosity due to the presence of the protein resid...
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Article
Open AccessEntangled polymer dynamics beyond reptation
Macroscopic properties of polymers arise from microscopic entanglement of polymer chains. Entangled polymer dynamics have been described theoretically by time- and space-averaged relaxation modes of single cha...
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Article
Open AccessConserved linear dynamics of single-molecule Brownian motion
Macromolecular diffusion in homogeneous fluid at length scales greater than the size of the molecule is regarded as a random process. The mean-squared displacement (MSD) of molecules in this regime increases l...
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Article
Open AccessControlling photophysical properties of ultrasmall conjugated polymer nanoparticles through polymer chain packing
Applications of conjugated polymer nanoparticles (Pdots) for imaging and sensing depend on their size, fluorescence brightness and intraparticle energy transfer. The molecular design of conjugated polymers (CP...
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Article
Open AccessSingle-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuations
Single-molecule localization and tracking has been used to translate spatiotemporal information of individual molecules to map their diffusion behaviours. However, accurate analysis of diffusion behaviours and...
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Article
A general approach to break the concentration barrier in single-molecule imaging
A method for single-molecule imaging at high protein concentrations is presented and demonstrated in the context of DNA replication in cell extracts.
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Article
Conformation and physics of polymer chains: a single-molecule perspective
Single-molecule optical detection and spectroscopy has emerged in recent years as an excellent tool for structural and dynamic characterization of materials and biological systems on nanometer scales. Measurin...
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Article
Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching
Recently, reversible photoswitching in bulk samples or in individual molecules of Dronpa, a mutant of a green fluorescent protein (GFP)-like fluorescent protein, has been demonstrated. Intense irradiation at 4...
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Single-Molecule Surface-Enhanced Resonance Raman Spectroscopy of the Enhanced Green Fluorescent Protein EGFP
We have investigated SM-SERRS spectra and intensity trajectories of individual EGFP molecules adsorbed on silver colloids. Spectral jumps, in a timescale of seconds, were observed in the SM-SERRS spectral traj...
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Article
Energy Gap Dependence of the Nonradiative Decay Rate Constant of 1-Anilino-8-naphthalene Sulfonate in Reverse Micelles
Steady-state and picosecond time-resolved fluorescence spectroscopies were conducted to study excited state properties of 1-anilino-8-naphthalene sulfonate (ANS) in reverse AOT (sodium bis(2-ethylhexyl)sulfosu...
