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Article
Control of DNA replication in vitro using a reversible replication barrier
A major obstacle to studying DNA replication is that it involves asynchronous and highly delocalized events. A reversible replication barrier overcomes this limitation and allows replication fork movement to b...
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Article
Replication fork uncoupling causes nascent strand degradation and fork reversal
Genotoxins cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial for genome stability and are exploited by chemotherapeutic approaches. However, it ...
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Protocol
Correction to: Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts
The chapter was inadvertently published with the Acknowledgment section excluded in the chapter.
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Protocol
Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts
DNA replication is crucial for cell viability and genome integrity. Despite its crucial role in genome duplication, the final stage of DNA replication, which is termed termination, is relatively unexplored. Ou...
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Article
Mechanisms of DNA replication termination
Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessa...
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Article
The mechanism of DNA replication termination in vertebrates
Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge. Termination involves local completion of DNA synthesis, decatenation of daughter molecules and replisome disass...
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Article
DNA interstrand cross-link repair requires replication-fork convergence
New biochemical analyses in Xenopus cell-free extracts show that two replication forks must converge on a DNA interstrand cross-link (ICL) to permit translesion synthesis and repair.
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Article
Similarities and differences between “uncapped” telomeres and DNA double-strand breaks
Telomeric DNA is present at the ends of eukaryotic chromosomes and is bound by telomere “cap**” proteins, which are the (Cdc13–Stn1–Ten1) CST complex, Ku (Yku70–Yku80), and Rap1–Rif1–Rif2 in budding yeast. I...
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Protocol
Simple, Non-radioactive Measurement of Single-Stranded DNA at Telomeric, Sub-telomeric, and Genomic Loci in Budding Yeast
Single-stranded DNA (ssDNA) is a DNA repair, replication, and recombination intermediate and a stimulus for checkpoint kinase-dependent cell cycle arrest. Current assays to detect ssDNA generated in vivo are i...
