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Article
Open AccessThe bacterial replication origin BUS promotes nucleobase capture
Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated...
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Article
Open AccessMultivalent interactions essential for lentiviral integrase function
A multimer of retroviral integrase (IN) synapses viral DNA ends within a stable intasome nucleoprotein complex for integration into a host cell genome. Reconstitution of the intasome from the maedi-visna virus...
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Article
Open AccessThree-dimensional super-resolution fluorescence imaging of DNA
Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined b...
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Article
Open AccessDuplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
A ring-shaped helicase unwinds DNA during chromosome replication in all organisms. Replicative helicases generally unwind duplex DNA an order of magnitude slower compared to their in vivo replication fork rate...
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Article
Open AccessThe mechanism of DNA unwinding by the eukaryotic replicative helicase
Accurate DNA replication is tightly regulated in eukaryotes to ensure genome stability during cell division and is performed by the multi-protein replisome. At the core an AAA+ hetero-hexameric complex, Mcm2-7...
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Prereplication-complex formation: a molecular double take?
DNA replication begins with prereplication-complex formation at origins and is followed by helicase activation to unwind DNA at the replication fork. This Perspective compares bacterial DnaB and eukaryotic MCM...
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Bypass of a protein barrier by a replicative DNA helicase
Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (known as steric exclusion). By contra...
