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Article
Open AccessBRD4 promotes resection and homology-directed repair of DNA double-strand breaks
Double-strand breaks (DSBs) are one of the most toxic forms of DNA damage and represent a major source of genomic instability. Members of the bromodomain and extra-terminal (BET) protein family are characteriz...
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Article
Octanol–Water Partition Coefficients of Aristolochic Acids and Implications to the Etiology of Balkan Endemic Nephropathy
The octanol–water partition coefficients (Kow) of the aristolochic acids, AA I and AA II, were determined using the traditional shake-flask method as a function of pH and ionic strength. These compounds have been...
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Article
Open AccessUncoupling of p97 ATPase activity has a dominant negative effect on protein extraction
p97 is a highly abundant, homohexameric AAA+ ATPase that performs a variety of essential cellular functions. Characterized as a ubiquitin-selective chaperone, p97 recognizes proteins conjugated to K48-linked p...
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Protocol
Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts
Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins wi...
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Article
Examination of the influence of dissolved halite (NaCl) on the leaching of lead (Pb) from cement-based solidified wastes
The leaching of lead from cement-based solidified waste forms mixed at different water/cement ratios was studied by conducting equilibrium and semi-dynamic leaching tests using deionized water and sodium chlor...
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Article
Exploring dissolved organic carbon cycling at the stream–groundwater interface across a third-order, lowland stream network
The stream–groundwater interface (SGI) is thought to be an important location within stream networks for dissolved organic carbon (DOC) processing (e.g., degradation, removal), since it is considered a hotspot...
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Protocol
Construction of Plasmids Containing Site-Specific DNA Interstrand Cross-Links for Biochemical and Cell Biological Studies
Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for prepara...
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Article
Evaluation of the hypothesis that Balkan endemic nephropathy is caused by drinking water exposure to contaminants leaching from Pliocene coal deposits
Balkan endemic nephropathy (BEN) is a kidney disease that has been reported in only certain rural villages in Serbia, Bulgaria, Romania, Croatia and Bosnia. The cause of BEN remains a mystery, but researchers ...
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Article
Nitrate concentrations and nitrate reduction in acid groundwater/lake systems in southern Australia
We have analyzed nitrate in saline groundwaters from the Lake Tyrrell region of north-western Victoria and the Norseman-Salmon Gums region of south-eastern Western Australia. These are regions where acid (pH<4...
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Chapter
Volume and activity quantitation in SPECT
This chapter reviews some of the factors which influence the quantitation of volume and activity with SPECT imaging. For volume quantitation we first compare seven methods of volume quantitation, and then illu...
