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7,348 Result(s)
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Article
Adenylate kinase fromRhodospirillum rubrum
A partial purification and some properties of adenylate kinase from the photosynthetic bacteriumRhodospirillum rubrum are described.
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Erratum
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Article
Construction and packaging of pseudotype retrovirus containing human N-ras cDNA antisense sequence and its biological effects on human hepatoma cells
N-ras is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mRNA and probein level in hepatoma cells. In order to explore the biological roles of N-
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Processing Pisum sativum seed storage protein precursors in vitro
The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea (Pisum sativum) is complex, resulting from cleavage (processing) of precursors expressed from multiple copies of genes...
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Comparing the frequencies of restriction fragment length polymorphisms for dystrophin gene in Chinese with those from Japanese and Caucasian populations
The restriction fragment length polymorphisms distribution and frequency of dystrophin gene in Chinese were studied by using 14 subclones of the entire 14kb cDNA for the dystrophin as hybridization probes. All...
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Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion
DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to esti...
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Effects of r- HuEP O on the biophysical characteristics of erythrocyte membrane in patients with anemia of chronic renal failure
Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from t...
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Structural analysis of DMD gene and its clinical application in Chinese.I.Bgl II exon-containing fragment, RFLP and carrier detection
This article is one of the serial studies on the characteristics of the molecular structure for dystrophin gene in Chinese. By using the entire dystrophin cDNA (14 kb) as a probe, the number and RFLPs of Bgl I...
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The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant-free condition
Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL /6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovalbumin(OVA), wh...
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High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery (EPD)
The production of transgenic swine for xenotransplantation has been proposed as an optimal option to overcome the chronic shortage of human organ donors. Generation of genetically engineered swine has been elu...
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A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins
Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integrat...
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Article
An Arabidopsis embryonic lethal mutant with reduced expression of alanyl-tRNA synthetase gene
In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identified and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell d...
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Interaction of viral proteins with host cell death machinery
In recent years, intense research has been directed towards understanding molecular mechanisms involved in viral pathogenesis. It is now known that many viruses manipulate host defense mechanisms to prevent ap...
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A possible hydrolysis mechanism of β-naphthyl acetate catalyzed by antibodies
The mechanism of ester hydrolysis has been extensively studied; however, the precise function of active-site residues in promoting catalysis is unclear. We describe here the structural models for the complex o...
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The kinetics of IL-4 and IFN-γ gene expression in Mice after Trichosansin immunization
Trichosanthin (TCS) is a potent allergen to mice. According to our previous experiments, it could bring out the IgE response to ovabumin (OVA) if TCS was given one day before OVA immunization, while OVA alone ...
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Construction of a DNA library from chromosome 4 of rice (Oryza sativa) by microdissection
A simple method to create a chromosome-specific DNA library of rice, including microdissection, amplification, characterization and cloning, is described. Rice chromosome 4 from a metaphase cell has been isola...
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Comparative investigation on spindle behavior and MPF activity changes during oocyte maturation between gynogenetic and amphimictic crucian carp
The spindle behavior and MPF activity changes in the progression of oocyte maturation were investigated and compared with cytological observation and kinase assay between gynogenetic silver crucian carp and am...
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Bacterial death induced by expression of the intracellular portion of human Fas
In attempting to produce the intracellular portion of human Fas (IC175–319) as a GST-fusion protein we found that expression of GST-IC175–319, but not GST alone or GST-IC231–298 (containing the Fas death domain),...
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Cloning and analysing of 5′ flanking region of Xenopus organizer gene noggin
Xenopus organizer specific gene Noggin possesses nearly all the characterestic properties of the action of organizer to specify the embryonic body axis. To analyze how the maternal inherited factors control it...
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Establishment and characterization of a caprine mammary epithelial cell line (CMEC)
We describe the establishment of a continuous, nontransformed cell line obtained from primary culture of a lactating (114 days postparturition) Anglo-Nubian (Capra hircus) goat mammary gland biopsy. These cells (...