19 Result(s)

Article

The genetic control of seed quality traits: effects of allelic variation at the Tri and Vc-2 genetic loci in Pisum sativum L.

Genetic variation at the Tri locus, controlling seed trypsin inhibitor activity, is relevant to both food and feed uses of Pisum sativum L. (pea). Near-isogenic lines of Pisum sativum L. (pea) were developed prev...

C. Chinoy, T. Welham, L. Turner, C. Moreau, C. Domoney in Euphytica (2011)

Article

Combinatorial variation in coding and promoter sequences of genes at the Tri locus in Pisum sativum accounts for variation in trypsin inhibitor activity in seeds

Cultivars of Pisum sativum that differ with respect to the quantitative expression of trypsin/chymotrypsin inhibitor proteins in seeds have been examined in terms of the structure of the corresponding genes. The...

D. Page, G. Aubert, G. Duc, T. Welham, C. Domoney in Molecular Genetics and Genomics (2002)

Article

Analysis of a lipoxygenase pseudogene in Pisum

 Lipoxygenase (LOX) enzymes play important roles in plant biology, and in the quality of plant-derived foods, through the production of fatty acid hydroperoxides that are metabolized either to jasmonate, or to...

C. Forster, C. Domoney, R. Casey in Theoretical and Applied Genetics (1999)

Chapter

Inhibitors of Legume Seeds

Enzyme inhibitors are prevalent among many plant species and have been detected in many different plant organs. Plant proteins have been identified that inhibit many diverse enzymes, including animal digestive...

C. Domoney in Seed Proteins (1999)

Chapter and Conference Paper

The Organization and Expression of Pea Seed Lipoxygenase Genes; Implications for Off-flavor Production in Frozen Peas and Pea Protein Isolates

A pea line that lacks seed lipoxygenase-2 was used to produce germplasm that may be commercially useful in the production of frozen peas and/or pea protein isolates because it produces less n-hexanal, an off-flav...

R. Casey, C. Domoney, C. Forster, M. O’Neill, Z. Wu… in Plant Proteins from European Crops (1998)

Article

Inheritance of qualitative and quantitative trypsin inhibitor variants in Pisum

A trypsin inhibitor locus (Tri) has been mapped close to Vc-2 on Pisum (pea) linkage group 5 using recombinant inbred lines derived from crosses of genotypes showing qualitative variation in seed trypsin inhibito...

C. Domoney, T. Welham, N. Ellis, R. Hellens in Theoretical and Applied Genetics (1994)

Article

Pisum lipoxygenase genes

The copy number, genomic arrangement and linkage relationships of two classes of lipoxygenase gene have been investigated in Pisum(pea) lines. Each of the two classes contained two to three members in P. sativum ...

C. Domoney, R. Casey, L. Turner, N. Ellis in Theoretical and Applied Genetics (1991)

Article

Another class of vicilin gene in Pisum

We describe a novel class of vicilin gene in Pisum corresponding to a precursor polypeptide of M_r 68000. The mRNA corresponding to this polypeptide accumulates during seed development in a pattern similar to that...

C. Domoney, R. Casey in Planta (1990)

Article

Lipoxygenase heterogeneity in Pisum sativum

Antibodies raised against two pea (Pisum sativum L. cv. Birte) seed lipoxygenases have been used to analyze lipoxygenase heterogeneity in seeds and in other organs. At least seven different polypeptides were iden...

C. Domoney, J. L. Firmin, C. Sidebottom, P. M. Ealing, A. Slabas, R. Casey in Planta (1990)

Article

Purification and partial characterisation of two abscisic-acid-responsive proteins induced in cultured embryos ofPisum sativum L.

When pea (Pisum sativum L.) embryos were cultured on low osmotica, with or without added abscisic acid (ABA), there was very little change in the total mRNA translation products resolved by one-dimensional sodium...

D. H. P. Barratt, C. Domoney, T. L. Wang in Planta (1989)

Article

Inheritance and map** of seed lipoxygenase polypeptides in Pisum

Analysis of crosses of Pisum lines showing variation in the apparent molecular weight of seed lipoxygenase polypeptides indicates that the genes encoding the two major pea seed lipoxygenase polypeptides are close...

H. North, R. Casey, C. Domoney in Theoretical and Applied Genetics (1989)

Article

Legumin heterogeneity inPisum

Analyses of heterogeneity in legumin subunit patterns, legumin precursor polypeptides, and restriction fragments containing legumin genes have shown thatPisum (pea) genotypes vary in the extent of gene and polype...

J. F. March, C. Domoney, R. Casey in Biochemical Genetics (1987)

Article

The structure of plant storage protein genes

R. Casey, C. Domoney in Plant Molecular Biology Reporter (1987)

Article

Changes in legumin messenger RNAs throughout seed development in Pisum sativum L.

The patterns of accumulation of three classes of legumin mRNA from Pisum sativum have been followed through seed development by cell-free translation and hybridization to complementary DNAs. Maximum amounts of mR...

C. Domoney, R. Casey in Planta (1987)

Article

Cloning and characterization of complementary DNA for convicilin, a major seed storage protein in Pisum sativum L.

A complementary DNA (cDNA) clone for convicilin, a major storage protein, has been isolated from a cDNA library prepared in the plasmid vector pAT 153, using poly(A)⁺RNA from develo** seeds of Pisum sativum L. ...

C. Domoney, R. Casey in Planta (1983)

Article

The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro

A highly sensitive immunoassay has been used for the detection of a major storage protein, legumin, in embryos of Pisum sativum L.; with this technique nanogram quantities could be measured. In the two varieties ...

C. Domoney, D. R. Davies, R. Casey in Planta (1980)