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Article
Tissue engineering of blood vessels with endothelial cells differentiated from mouse embryonic stem cells
Endothelial cells (TEC3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8×106 smooth muscle cells (SMCs...
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Article
The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine
As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment ...
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Article
The developmental fate of green fluorescent mouse embryonic germ cells in chimeric embryos
Primordial germ cells (PGCs), as precursors of mammalian germ lineage, have been gaining more attention as a new resource of pluripotent stem cells, which bring a great possibility to study developmental event...
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Article
A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins
Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integrat...
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Article
Can overexpression of TGF-β1 gene change the sex ratio in transgenic mice?
Mouse TGF-β1 gene was microinjected into male pronuclei of F2 hybrid fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to generate transgenic mice with over-expressed TGF-β1 gene. The rate of ...
