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Article
The nuclear receptor LXR is a glucose sensor
The liver has a central role in glucose homeostasis, as it has the distinctive ability to produce and consume glucose1. On feeding, glucose influx triggers gene expression changes in hepatocytes to suppress endog...
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Chapter
Sterol–Protein Interactions in Cholesterol and Bile Acid Synthesis
Cholesterol and other cholesterol related metabolites, oxysterols, and bile acids, establish specific interactions with enzymes and other proteins involved in cholesterol and bile acid homeostasis, triggering ...
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Protocol
Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators
The capacity to induce the association of peroxisome proliferator-activated receptors (PPARs) with different transcriptional coregulators is determined by the peculiar 3D-structure that the receptors adopt whe...
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Protocol
Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As ...
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Article
Open AccessThe lipogenic regulator Sterol Regulatory Element Binding Factor-1c is required to maintain peripheral nerve structure and function
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Article
Open AccessLiver ERα regulates AgRP neuronal activity in the arcuate nucleus of female mice
Recent work revealed the major role played by liver Estrogen Receptor α (ERα) in the regulation of metabolic and reproductive functions. By using mutant mice with liver-specific ablation of Erα, we here demonstra...
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Article
Open AccessThe ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cells
Vγ9Vδ2 T cells are activated by phosphoantigens, such as isopentenyl pyrophosphate (IPP), which is generated in the mevalonate pathway of antigen-presenting cells. IPP is released in the extracellular microenv...
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Article
Open AccessHDAC3 is a molecular brake of the metabolic switch supporting white adipose tissue browning
White adipose tissue (WAT) can undergo a phenotypic switch, known as browning, in response to environmental stimuli such as cold. Post-translational modifications of histones have been shown to regulate cellul...
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Article
Open AccessAxonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features
Disruption of axonal transport plays a pivotal role in diabetic neuropathy. A sex-dimorphism exists in the incidence and symptomatology of diabetic neuropathy; however, no studies so far have addressed sex dif...
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Article
Open AccessMyeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation
Cholesterol homeostasis has a pivotal function in regulating immune cells. Here we show that apolipoprotein E (apoE) deficiency leads to the accumulation of cholesterol in the cell membrane of dendritic cells ...
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Article
Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP
Extracellular matrix (ECM) mechanical cues have powerful effects on cell proliferation, differentiation and death. Here, starting from an unbiased metabolomics approach, we identify synthesis of neutral lipids...
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Article
Open AccessDNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant buffering
Accumulation of DNA lesions causing transcription stress is associated with natural and accelerated aging and culminates with profound metabolic alterations. Our understanding of the mechanisms governing metab...
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Article
The oligosaccharide portion of ganglioside GM1 regulates mitochondrial function in neuroblastoma cells
The crucial role of ganglioside GM1 in the regulation of neural homeostasis has been assessed by several studies. Recently we shed new light on the molecular basis underlying GM1 effects demonstrating that GM1...
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Article
Metabolic control of DNA methylation in naive pluripotent cells
Naive epiblast and embryonic stem cells (ESCs) give rise to all cells of adults. Such developmental plasticity is associated with genome hypomethylation. Here, we show that LIF–Stat3 signaling induces genomic ...
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Article
Open AccessCa2+ overload- and ROS-associated mitochondrial dysfunction contributes to δ-tocotrienol-mediated paraptosis in melanoma cells
Melanoma is an aggressive tumor with still poor therapy outcomes. δ-tocotrienol (δ-TT) is a vitamin E derivative displaying potent anti-cancer properties. Previously, we demonstrated that δ-TT triggers apoptos...
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Article
The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites
White to brown/beige adipocytes conversion is a possible therapeutic strategy to tackle the current obesity epidemics. While mitochondria are key for energy dissipation in brown fat, it is unknown if they can ...
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Article
Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition
Vascular mural cells (vMCs) play an essential role in the development and maturation of the vasculature by promoting vessel stabilization through their interactions with endothelial cells. Whether endothelial ...
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Article
Author Correction: The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites
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Article
Open AccessMetabolomic signature and mitochondrial dynamics outline the difference between vulnerability and resilience to chronic stress
Stress is the foremost environmental factor involved in the pathophysiology of major depressive disorder (MDD). However, individual differences among people are critical as some people exhibit vulnerability wh...
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Article
Open AccessChanges in the lipidome of water buffalo milk during intramammary infection by non-aureus Staphylococci
This study aimed to determine the lipidome of water buffalo milk with intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci, using an untargeted lipid...
