124 Result(s)
within
Teruo Okano
English
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Article
Open AccessUmbilical cord-derived mesenchymal stem cell sheets transplanted subcutaneously enhance cell retention and survival more than dissociated stem cell injections
Human umbilical cord-derived mesenchymal stem cell (hUC-MSC) sheets have recently attracted attention as an alternative approach to injected cell suspensions for stem cell therapy. However, cell engraftment an...
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Article
Open AccessRapid and effective preparation of clonal bone marrow-derived mesenchymal stem/stromal cell sheets to reduce renal fibrosis
Allogeneic “off-the-shelf” mesenchymal stem/stromal cell (MSC) therapy requires scalable, quality-controlled cell manufacturing and distribution systems to provide clinical-grade products using cryogenic cell ...
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Article
Open AccessPolydactyly-derived allogeneic chondrocyte cell-sheet transplantation with high tibial osteotomy as regenerative therapy for knee osteoarthritis
Allogeneic cell therapies are not fully effective in treating osteoarthritis of the knee (OAK). We recently reported that transplantation of autologous chondrocyte cell-sheets along with open-wedge high tibial...
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Protocol
Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure
This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of smal...
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Article
Open AccessSafety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment
Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold...
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Article
Open Access3D cell sheet structure augments mesenchymal stem cell cytokine production
Mesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during tissue regeneration. An increasing body of evidence suggests that this paracrine function is enhanced by MSC cultivation i...
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Article
Open AccessImprovement of the therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet
Although pancreatic islet transplantation therapy is ideal for diabetes patients, several hurdles have prevented it from becoming a standard treatment, including donor shortage and low engraftment efficacy. In...
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Reference Work Entry In depth
Cell Sorting, Culture, Preconditioning, and Modulation/Cell Aggregates: Sheets
A novel cell culture surface grafted with the temperature-responsive polymer poly(N-isopropylacrylamide) has been developed for controlled attachment and detachment of living cells through temperature changes. Th...
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Article
Open AccessFabrication of hyaline-like cartilage constructs using mesenchymal stem cell sheets
Cell and tissue engineering approaches for articular cartilage regeneration increasingly focus on mesenchymal stem cells (MSCs) as allogeneic cell sources, based on availability and innate chondrogenic potenti...
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Article
Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip
In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections betwe...
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Article
Open AccessPhenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets
In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inco...
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Article
Open AccessHuman mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Cell-based therapies are increasingly focused on allogeneic stem cell sources because of several advantages in eliminating donor variability (e.g., aging and disease pathophysiology) affecting stem cell qualit...
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Article
Open AccessUsing cell sheets to regenerate mouse submandibular glands
Temperature-responsive polymer grafted tissue culture dishes release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining cell–cell communications,...
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Article
Open AccessCombined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis
Current cartilage regenerative therapies are not fully effective in treating osteoarthritis of the knee (OAK). We have developed chondrocyte sheets for autologous transplantation and tested these in in vitro a...
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Article
Open AccessEngineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
Skeletal muscle physiology and the mechanisms of muscle diseases can be effectively studied by an in-vitro tissue model produced by muscle tissue engineering. Engineered human cell-based tissues are required more...
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Article
Open AccessEndoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats
Owing to the recent progress in regenerative medicine technology, clinical trials that harnessed the regeneration and immune modulation potentiality of stem cells for treating IBD have shown promising results....
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Article
Open AccessEngineered mesenchymal stem-cell-sheets patches prevents postoperative pancreatic leakage in a rat model
Post-operative pancreatic fistula (POPF) following pancreatic resection is a life-threatening surgical complication. Cell sheets were prepared and harvested using temperature-responsive culture dishes and tran...
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Living Reference Work Entry In depth
Cell Sorting, Culture, Preconditioning, and Modulation/Cell Aggregates: Sheets
A novel cell culture surface grafted with the temperature-responsive polymer poly(N-isopropylacrylamide) has been developed for controlled attachment and detachment of living cells through temperature changes. Th...
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Chapter
Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet-Based Regenerative Therapy and 3D Tissue Fabrication
This chapter describes the concept of “cell sheet engineering” for the creation of transplantable cellular tissues and organs. In contrast to scaffold-based tissue engineering, cell sheet engineering facilitat...
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Article
Open AccessOral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation
Endoscopic submucosal dissection (ESD) permits en bloc removal of superficial oesophageal squamous cell carcinoma (ESCC). However, post-procedure stricture is common after ESD for widespread tumours, and multiple...
