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Open AccessDual-color FISH analyses of xenogeneic human fibroblast sheets transplanted to repair lung pleural defects in an immunocompromised rat model
Pulmonary air leaks (PALs) due to visceral pleura injury during surgery is frequently observed after pulmonary resections and the complication is difficult to avoid in thoracic surgery. The development of post...
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Article
Application of mesenchymal stromal cell sheets to prevent medication-related osteonecrosis of the jaw with titanium implants in rats
Medication-related osteonecrosis of the jaw (MRONJ) is an intractable adverse event. Dental implants are one of the triggering factors of MRONJ, and implant therapy with low MRONJ risk is required. This study ...
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Article
Open AccessPolydactyly-derived allogeneic chondrocyte cell-sheet transplantation with high tibial osteotomy as regenerative therapy for knee osteoarthritis
Allogeneic cell therapies are not fully effective in treating osteoarthritis of the knee (OAK). We recently reported that transplantation of autologous chondrocyte cell-sheets along with open-wedge high tibial...
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Article
Open AccessCytomegalovirus‐associated esophagitis on early esophageal cancer in immunocompetent host: a case report
Cytomegalovirus (CMV)-associated gastrointestinal diseases usually occur in immunocompromised patients; however, few cases has also been described in healthy hosts despite still unclear pathological mechanisms...
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Article
Open AccessBio-artificial pleura using autologous dermal fibroblast sheets to mitigate air leaks during thoracoscopic lung resection
Lung air leaks (LALs) due to visceral pleura injury during surgery are a difficult-to-avoid complication in thoracic surgery (TS). Reliable LAL closure is an important patient management issue after TS. We dem...
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Article
Open AccessCombined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis
Current cartilage regenerative therapies are not fully effective in treating osteoarthritis of the knee (OAK). We have developed chondrocyte sheets for autologous transplantation and tested these in in vitro a...
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Article
Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells
The extracellular matrix (ECM) is a major biomechanical environment for all cells in vivo, and tightly controls wound healing and cancer progression. Type I collagen (Col I) is the most abundant component in E...
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Article
Silibinin-induced autophagy mediated by PPARα-sirt1-AMPK pathway participated in the regulation of type I collagen-enhanced migration in murine 3T3-L1 preadipocytes
Preadipocyte migration is a fundamental and important process for the development of tissue organization, especially in the development of primitive adipose tissue and adipocyte tissue wound healing. However, ...
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Article
Enhanced migration of murine fibroblast-like 3T3-L1 preadipocytes on type I collagen-coated dish is reversed by silibinin treatment
Migration of fibroblast-like preadipocytes is important for the development of adipose tissue, whereas excessive migration is often responsible for impaired adipose tissue related with obesity and fibrotic dis...
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Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet-Based Regenerative Therapy and 3D Tissue Fabrication
This chapter describes the concept of “cell sheet engineering” for the creation of transplantable cellular tissues and organs. In contrast to scaffold-based tissue engineering, cell sheet engineering facilitat...
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Open AccessOral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation
Endoscopic submucosal dissection (ESD) permits en bloc removal of superficial oesophageal squamous cell carcinoma (ESCC). However, post-procedure stricture is common after ESD for widespread tumours, and multiple...
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Article
Open AccessBio-artificial pleura using an autologous dermal fibroblast sheet
Air leaks (ALs) are observed after pulmonary resections, and without proper treatment, can produce severe complications. AL prevention is a critical objective for managing patients after pulmonary resection. T...
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Article
Open AccessMiddle ear mucosal regeneration by tissue-engineered cell sheet transplantation
The recurrence of cholesteatoma after surgical treatment often occurs as a result of poor mucosal regeneration in the middle ear cavity and mastoid cavity and changes, such as granulation tissue formation, whi...
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Article
Retraction Note: Reproducible subcutaneous transplantation of cell sheets into recipient mice
Nat. Protoc. 6, 1053–1059 (2011); published online 30 June 2011; retracted 13 January 2016 Masayuki Yamato, Satoshi Tsuneda and Teruo Okano would like to retract this protocol after concerns were raised by the...
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Cell Sheets for Periodontal Tissue Engineering
Cell Sheet Technology was first utilized in a clinical trial for corneal reconstruction in 2003; since then, several clinical trials have been performed not only in Japan but also in Europe. The key technology...
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Measurement of the dynamic behavior of thin poly(N-isopropylacrylamide) hydrogels and their phase transition temperatures measured using reflectometric interference spectroscopy
Temperature-responsive cell culture surfaces prepared by modifying tissue-culture polystyrene with nanoscale poly(N-isopropylacrylamide) (PIPAAm) hydrogels are widely used as intelligent surfaces for the fabricat...
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Sociocytology Illuminated by Reconstructing Functional Tissue with Cell Sheet Based Technology
To create cell-dense functional tissues, the authors have developed a new class of tissue reconstructing technology, called “cell-sheet engineering”. In contrast to biodegradable scaffold-based three-dimension...
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Article
Control of Cell Adhesion and Detachment on Temperature-Responsive Block Copolymer Langmuir Films
This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Block copolymer composed of polystyrene (PSt) and PIPAAm was synthesized by RAFT p...
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Article
Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation
Thready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and det...
