15 Result(s)
within
Katsuhisa Sakaguchi
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Article
Metallic Vessel with Mesh Culture Surface Fabricated Using Three-dimensional Printing Engineers Tissue Culture Environment
Various culture devices have been developed as fundamental technologies for facilitating bioengineering studies. Culture devices are designed to prepare specific culture environments. Thus, both macrostructure...
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Article
Open AccessProduction of scaffold-free cell-based meat using cell sheet technology
In the production of cell-based meat, it is desirable to reduce animal-derived materials as much as possible to meet the challenges of sustainability. Here, we demonstrate the “cell sheet-based meat”: scaffold...
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Article
Open AccessIn vitro ballooned hepatocytes can be produced by primary human hepatocytes and hepatic stellate cell sheets
Despite the increasing prevalence of Nonalcoholic steatohepatitis (NASH) worldwide, there is no effective treatment available for this disease. “Ballooned hepatocyte” is a characteristic finding in NASH and is...
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Article
Functional Analysis of Induced Human Ballooned Hepatocytes in a Cell Sheet-Based Three Dimensional Model
Ballooned hepatocytes (BH) are a key histological hallmark of nonalcoholic steatohepatitis (NASH), yet their consequences for liver-specific functions are unknown.
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Chapter and Conference Paper
Reconstruction of a Vascular Bed with Perfusable Blood Vessels Using a Decellularized Porcine Small Intestine for Clinical Application
Engineering three-dimensional tissues in vitro for regenerative therapy is highly desired. Vascular beds with connectable arteries and veins play crucial roles in fabricating three-dimensional tissues in vitro an...
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Article
Printing support hydrogels for creating vascular-like structures in stacked cell sheets
Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few h...
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Article
Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip
In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections betwe...
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Article
Simple action potential measurement of cardiac cell sheet utilizing electronic sheet
In this study, a device for measuring the action potential of cardiac cell sheets was developed. The action potential was measured using a device comprising a 2-µm-thick parylene film with a silver electrode p...
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Article
Fundamental characteristics of printed gelatin utilizing micro 3D printer
Gelatin is useful for biofabrication, because it can be used for cell scaffolds and it has unique properties. Therefore, we attempted to fabricate biodevices of gelatin utilizing micro 3D printer which is able...
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Article
Fabrication of micro-gelatin fiber utilizing coacervation method
Biotechnology has drastically been advanced by the development of iPS and ES cells, which are representative forms induced pluripotent stem cells. In the micro/nano bio field, the development of cells and Tayl...
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Article
Open AccessThicker three-dimensional tissue from a “symbiotic recycling system” combining mammalian cells and algae
In this paper, we report an in vitro co-culture system that combines mammalian cells and algae, Chlorococcum littorale, to create a three-dimensional (3-D) tissue. While the C2C12 mouse myoblasts and rat cardiac ...
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Article
Open AccessIn Vitro Engineering of Vascularized Tissue Surrogates
In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissu...
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Article
Open AccessIn vitro fabrication of functional three-dimensional tissues with perfusable blood vessels
Artificially engineered tissues may have many therapeutic applications but complex tissues are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with pe...
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Article
Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro
The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the...
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Chapter and Conference Paper
Fabrication of Three-Dimensional Tissues with Perfused Microchannels
Recently, researchers have challenged to create three-dimensional (3-D) tissues with tissue engineering technology in order to establish in vitro models and new therapy for damaged organ. We have developed cel...
